共 15 条
Fluostatins Produced by the Heterologous Expression of a TAR Reassembled Environmental DNA Derived Type II PKS Gene Cluster
被引:78
作者:

Feng, Zhiyang
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Genetically Encoded Small Mol, Howard Hughes Med Inst, New York, NY 10065 USA Rockefeller Univ, Lab Genetically Encoded Small Mol, Howard Hughes Med Inst, New York, NY 10065 USA

Kim, Jeff H.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Genetically Encoded Small Mol, Howard Hughes Med Inst, New York, NY 10065 USA Rockefeller Univ, Lab Genetically Encoded Small Mol, Howard Hughes Med Inst, New York, NY 10065 USA

Brady, Sean F.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Genetically Encoded Small Mol, Howard Hughes Med Inst, New York, NY 10065 USA Rockefeller Univ, Lab Genetically Encoded Small Mol, Howard Hughes Med Inst, New York, NY 10065 USA
机构:
[1] Rockefeller Univ, Lab Genetically Encoded Small Mol, Howard Hughes Med Inst, New York, NY 10065 USA
关键词:
TRANSFORMATION-ASSOCIATED RECOMBINATION;
DIVERSITY;
BIOSYNTHESIS;
CLONING;
D O I:
10.1021/ja104550p
中图分类号:
O6 [化学];
学科分类号:
0703 ;
摘要:
Culture independent approaches for accessing small molecules produced by uncultured bacteria are often hampered by the inability to easily clone environmental DNA (eDNA) fragments large enough to capture intact biosynthetic gene clusters that can be used in heterologous expression studies. Here we show that homology screening of eDNA megalibraries for clones containing natural product biosynthetic genes, coupled with transformation-assisted recombination (TAR) in yeast, can be used to access large, functionally intact, natural product gene clusters from the environment. The eDNA derived gene cluster reported here was functionally reconstructed from two overlapping cosmid clones using TAR. The isolation and structure elucidation of three new fluostatins (F, G, and H) produced by this TAR reconstructed gene cluster is described.
引用
收藏
页码:11902 / 11903
页数:2
相关论文
共 15 条
[1]
Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules
[J].
Brady, Sean F.
.
NATURE PROTOCOLS,
2007, 2 (05)
:1297-1305

Brady, Sean F.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Genet Encoded Small Mol, New York, NY 10021 USA Rockefeller Univ, Lab Genet Encoded Small Mol, New York, NY 10021 USA
[2]
The Biosynthetic Logic of Polyketide Diversity
[J].
Hertweck, Christian
.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,
2009, 48 (26)
:4688-4716

论文数: 引用数:
h-index:
机构:
[3]
BIOSYNTHESIS OF VINEOMYCIN-A1 AND VINEOMYCIN-B2
[J].
IMAMURA, N
;
KAKINUMA, K
;
IKEKAWA, N
;
TANAKA, H
;
OMURA, S
.
JOURNAL OF ANTIBIOTICS,
1982, 35 (05)
:602-608

IMAMURA, N
论文数: 0 引用数: 0
h-index: 0
机构: KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN

KAKINUMA, K
论文数: 0 引用数: 0
h-index: 0
机构: KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN

IKEKAWA, N
论文数: 0 引用数: 0
h-index: 0
机构: KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN

TANAKA, H
论文数: 0 引用数: 0
h-index: 0
机构: KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN

OMURA, S
论文数: 0 引用数: 0
h-index: 0
机构: KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN
[4]
Cloning Large Natural Product Gene Clusters from the Environment: Piecing Environmental DNA Gene Clusters Back Together with TAR
[J].
Kim, Jeffrey H.
;
Feng, Zhiyang
;
Bauer, John D.
;
Kallifidas, Dimitris
;
Calle, Paula Y.
;
Brady, Sean F.
.
BIOPOLYMERS,
2010, 93 (09)
:833-844

Kim, Jeffrey H.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA

Feng, Zhiyang
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA

Bauer, John D.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA

Kallifidas, Dimitris
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA

Calle, Paula Y.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA

Brady, Sean F.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA Rockefeller Univ, Howard Hughes Med Inst, Lab Genetically Encoded Small Mol, New York, NY 10065 USA
[5]
An Environmental DNA-Derived Type II Polyketide Biosynthetic Pathway Encodes the Biosynthesis of the Pentacyclic Polyketide Erdacin
[J].
King, Ryan W.
;
Bauer, John D.
;
Brady, Sean F.
.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,
2009, 48 (34)
:6257-6261

King, Ryan W.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Genetically Encoded Small Mol, New York, NY 10065 USA Rockefeller Univ, Lab Genetically Encoded Small Mol, New York, NY 10065 USA

Bauer, John D.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Genetically Encoded Small Mol, New York, NY 10065 USA Rockefeller Univ, Lab Genetically Encoded Small Mol, New York, NY 10065 USA

Brady, Sean F.
论文数: 0 引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Genetically Encoded Small Mol, New York, NY 10065 USA Rockefeller Univ, Lab Genetically Encoded Small Mol, New York, NY 10065 USA
[6]
Highly selective isolation of human DNAs from rodent-human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning
[J].
Larionov, V
;
Kouprina, N
;
Graves, J
;
Resnick, MA
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1996, 93 (24)
:13925-13930

Larionov, V
论文数: 0 引用数: 0
h-index: 0
机构: Laboratory of Molecular Genetics, Natl. Inst. of Environ. Hlth. Sci., National Institutes of Health, Research Triangle Park, NC 27709

Kouprina, N
论文数: 0 引用数: 0
h-index: 0
机构: Laboratory of Molecular Genetics, Natl. Inst. of Environ. Hlth. Sci., National Institutes of Health, Research Triangle Park, NC 27709

Graves, J
论文数: 0 引用数: 0
h-index: 0
机构: Laboratory of Molecular Genetics, Natl. Inst. of Environ. Hlth. Sci., National Institutes of Health, Research Triangle Park, NC 27709

Resnick, MA
论文数: 0 引用数: 0
h-index: 0
机构: Laboratory of Molecular Genetics, Natl. Inst. of Environ. Hlth. Sci., National Institutes of Health, Research Triangle Park, NC 27709
[7]
TRANSFORMATION-ASSOCIATED RECOMBINATION BETWEEN DIVERGED AND HOMOLOGOUS DNA REPEATS IS INDUCED BY STRAND BREAKS
[J].
LARIONOV, V
;
KOUPRINA, N
;
ELDAROV, M
;
PERKINS, E
;
PORTER, G
;
RESNICK, MA
.
YEAST,
1994, 10 (01)
:93-104

LARIONOV, V
论文数: 0 引用数: 0
h-index: 0
机构: ST PETERSBURG CYTOL INST,ST PETERSBURG,RUSSIA

KOUPRINA, N
论文数: 0 引用数: 0
h-index: 0
机构: ST PETERSBURG CYTOL INST,ST PETERSBURG,RUSSIA

ELDAROV, M
论文数: 0 引用数: 0
h-index: 0
机构: ST PETERSBURG CYTOL INST,ST PETERSBURG,RUSSIA

PERKINS, E
论文数: 0 引用数: 0
h-index: 0
机构: ST PETERSBURG CYTOL INST,ST PETERSBURG,RUSSIA

PORTER, G
论文数: 0 引用数: 0
h-index: 0
机构: ST PETERSBURG CYTOL INST,ST PETERSBURG,RUSSIA

RESNICK, MA
论文数: 0 引用数: 0
h-index: 0
机构: ST PETERSBURG CYTOL INST,ST PETERSBURG,RUSSIA
[8]
ISOLATION, CHARACTERIZATION, AND STRUCTURE OF RABELOMYCIN, A NEW ANTIBIOTIC
[J].
LIU, WC
;
PARKER, WL
;
SLUSARCH.DS
;
GREENWOOD, GL
;
GRAHAM, SF
;
MEYERS, E
.
JOURNAL OF ANTIBIOTICS,
1970, 23 (09)
:437-+

LIU, WC
论文数: 0 引用数: 0
h-index: 0

PARKER, WL
论文数: 0 引用数: 0
h-index: 0

SLUSARCH.DS
论文数: 0 引用数: 0
h-index: 0

GREENWOOD, GL
论文数: 0 引用数: 0
h-index: 0

GRAHAM, SF
论文数: 0 引用数: 0
h-index: 0

MEYERS, E
论文数: 0 引用数: 0
h-index: 0
[9]
The uncultured microbial majority
[J].
Rappé, MS
;
Giovannoni, SJ
.
ANNUAL REVIEW OF MICROBIOLOGY,
2003, 57
:369-394

Rappé, MS
论文数: 0 引用数: 0
h-index: 0
机构:
Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA

Giovannoni, SJ
论文数: 0 引用数: 0
h-index: 0
机构:
Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA
[10]
The structures of fluostatins C, D and E, novel members of the fluostatin family
[J].
Schneider, K
;
Nicholson, G
;
Ströbele, M
;
Baur, S
;
Niehaus, J
;
Fiedler, HP
;
Süssmuth, RD
.
JOURNAL OF ANTIBIOTICS,
2006, 59 (02)
:105-109

Schneider, K
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Berlin, Inst Organ Chem, D-10623 Berlin, Germany

Nicholson, G
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Berlin, Inst Organ Chem, D-10623 Berlin, Germany

Ströbele, M
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Berlin, Inst Organ Chem, D-10623 Berlin, Germany

Baur, S
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Berlin, Inst Organ Chem, D-10623 Berlin, Germany

Niehaus, J
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Berlin, Inst Organ Chem, D-10623 Berlin, Germany

Fiedler, HP
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Berlin, Inst Organ Chem, D-10623 Berlin, Germany

Süssmuth, RD
论文数: 0 引用数: 0
h-index: 0
机构: Tech Univ Berlin, Inst Organ Chem, D-10623 Berlin, Germany