Ligand-induced dimerization of Drosophila peptidoglycan recognition proteins in vitro

被引:87
作者
Mellroth, P
Karlsson, J
Håkansson, J
Schultz, N
Goldman, WE
Steiner, H [1 ]
机构
[1] Univ Stockholm, Dept Genet Microbiol & Toxicol, S-10691 Stockholm, Sweden
[2] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA
关键词
Imd/Relish; innate immunity; pattern recognition; Toll/Dif;
D O I
10.1073/pnas.0407559102
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Drosophila knockout mutants have placed peptidoglycan recognition proteins (PGRPs) in the two major pathways controlling immune gene expression. We now examine PGRP affinities for peptidoglycan. PGRP-SA and PGRP-LCx are bona fide pattern recognition receptors, and PGRP-SA, the peptidoglycan receptor of the Toll/Dif pathway, has selective affinity for different peptidoglycans. PGRP-LCx, the default peptidoglycan receptor of the Imd/Relish pathway, has strong affinity for all polymeric peptidoglycans tested and for monomeric peptidoglycan. PGRP-LCa does not have affinity for polymeric or monomeric peptidoglycan. Instead, PGRP-LCa can form heterodimers with LCx when the latter is bound to monomeric peptidoglycan. Hence, PGRP-LCa can be said to function as an adaptor, thus adding a new function to a member of the PGRP family.
引用
收藏
页码:6455 / 6460
页数:6
相关论文
共 25 条
[1]   Toll-like receptor signalling [J].
Akira, S ;
Takeda, K .
NATURE REVIEWS IMMUNOLOGY, 2004, 4 (07) :499-511
[2]   Function of the drosophila pattern-recognition receptor PGRP-SD in the detection of Gram-positive bacteria [J].
Bischoff, V ;
Vignal, C ;
Boneca, IG ;
Michel, T ;
Hoffmann, JA ;
Royet, J .
NATURE IMMUNOLOGY, 2004, 5 (11) :1175-1180
[3]   A Drosophila pattern recognition receptor contains a peptidoglycan docking groove and unusual L,D-carboxypeptidase activity [J].
Chang, CI ;
Pili-Floury, S ;
Hervé, M ;
Parquet, C ;
Chelliah, Y ;
Lemaitre, B ;
Mengin-Lecreulx, D ;
Deisenhofer, J .
PLOS BIOLOGY, 2004, 2 (09) :1293-1302
[4]   NEW APPROACH TO CALCULATION OF SECONDARY STRUCTURES OF GLOBULAR PROTEINS BY OPTICAL ROTATORY DISPERSION AND CIRCULAR DICHROISM [J].
CHEN, YH ;
YANG, JT .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1971, 44 (06) :1285-&
[5]   Drosophila peptidoglycan recognition protein LC (PGRP-LC) acts as a signal-transducing innate immune receptor [J].
Choe, KM ;
Lee, H ;
Anderson, KV .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (04) :1122-1126
[6]   Requirement for a peptidoglycan recognition protein (PGRP) in relish activation and antibacterial immune responses in Drosophila [J].
Choe, KM ;
Werner, T ;
Stöven, S ;
Hultmark, D ;
Anderson, KV .
SCIENCE, 2002, 296 (5566) :359-362
[7]   BIOLOGICAL-ACTIVITIES AND CHEMICAL-COMPOSITION OF PURIFIED TRACHEAL CYTO-TOXIN OF BORDETELLA-PERTUSSIS [J].
COOKSON, BT ;
CHO, HL ;
HERWALDT, LA ;
GOLDMAN, WE .
INFECTION AND IMMUNITY, 1989, 57 (07) :2223-2229
[8]   Dual activation of the Drosophila Toll pathway by two pattern recognition receptors [J].
Gobert, V ;
Gottar, M ;
Matskevich, AA ;
Rutschmann, S ;
Royet, J ;
Belvin, M ;
Hoffmann, JA ;
Ferrandon, D .
SCIENCE, 2003, 302 (5653) :2126-2130
[9]   The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein [J].
Gottar, M ;
Gobert, V ;
Michel, T ;
Belvin, M ;
Duyk, G ;
Hoffmann, JA ;
Ferrandon, D ;
Royet, J .
NATURE, 2002, 416 (6881) :640-644
[10]   Structural basis for peptidoglycan binding by peptidoglycan recognition proteins [J].
Guan, RJ ;
Roychowdhury, A ;
Ember, B ;
Kumar, S ;
Boons, GJ ;
Mariuzza, RA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (49) :17168-17173