Production of 11α-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene

被引:13
作者
Ahmed, E. M. [1 ]
机构
[1] Natl Res Ctr, Nat & Microbial Prod Chem Dept, Cairo, Egypt
关键词
11; alpha-hydroxprogesterone; polytetrafluoroethylene; Aspergillus terreus;
D O I
10.1590/S1517-83822007000200007
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11 alpha-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30 degrees C. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4 degrees C for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles.
引用
收藏
页码:224 / 229
页数:6
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