Clinical significance of miR-221 and its inverse correlation with p27Kip1 in hepatocellular carcinoma

被引：65

作者：

Fu, Xinhui ^{[2
,3
]}

Wang, Qian ^{[1
]}

Chen, Jingsong ^{[1
]}

Huang, Xiaohui ^{[2
]}

Chen, Xilin ^{[1
]}

Cao, Liangqi ^{[1
]}

Tan, Haoxiang ^{[1
]}

Li, Wen ^{[2
]}

Zhang, Longjuan ^{[2
]}

Bi, Jiong ^{[2
]}

Su, Qiao ^{[2
]}

Chen, Lianzhou ^{[2
]}

机构：

[1] Sun Yat Sen Zhongshan Univ, Dept Hepatobiliary Surg, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Zhongshan Univ, Lab Surg, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China

[3] Sun Yat Sen Zhongshan Univ, Affiliated Hosp 6, Inst Gastrointestinal Dis, Guangzhou 510655, Guangdong, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2011年 / 38卷 / 05期

基金：

中国国家自然科学基金;

关键词：

HCC; In situ hybridization; miR-221; p27(Kip1); MICRORNA EXPRESSION;

D O I：

10.1007/s11033-010-9969-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The aim of the present study is to explore possible role of miR-221 in the pathogenesis of HCC. Matched HCC and adjacent non-cancerous samples were assayed for the expression of miR-221 and three G1/S transition inhibitors: p27(Kip1), p21(WAF1/Cip1)and TGF-beta 1 by in situ hybridization and immunohistochemistry respectively. p27(Kip1) is one of miR-221's proven targets. Real time qRT-PCR was used to investigate miR-221 and p27(Kip1) transcripts in different clinical stages. Western blotting was used to analyze the expression levels of p27(Kip1) protein in different clinical stages. In result, miR-221 and TGF-beta 1 are frequently up-regulated in HCC, while p27(Kip1) and p21(WAF1/Cip1) proteins are frequently down-regulated. Moreover, miR-221 and p27(Kip1)'s expression correlated with metastasis and miR-221's expression also correlated with tumor size. Both of p21(WAF1/Cip1)and TGF-beta 1's expression correlated with tumor differentiations. miR-221's upregulation and p27(Kip1)'s downregulation were significantly associated with tumor stages and metastasis. In conclusion, miR-221 is important in tumorigenesis of HCC, possibly by specifically down-regulating p27(Kip1), a cell-cycle inhibitor. These results indicate miR-221 as a new therapeutic target in HCC.

引用

页码：3029 / 3035

页数：7

共 17 条

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