Toxigenic status of Clostridium difficile in a large Spanish teaching hospital

被引:18
作者
Alonso, R [1 ]
Martín, A [1 ]
Peláez, T [1 ]
Marín, M [1 ]
Rodríguez-Creixéms, M [1 ]
Bouza, E [1 ]
机构
[1] Hosp Gen Univ Gregorio Maranon, Dept Clin Microbiol & Infect Dis, Madrid 28007, Spain
关键词
D O I
10.1099/jmm.0.45809-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The aim of this study was to evaluate the toxigenic status of circulating strains of Clostridium difficile in a large teaching hospital. Overall 220 isolates were studied of which 199 (90.5%) produced both large clostridial toxins detected by conventional methods. Ten more strains (4.5%) had toxin A and B genes detectable by PCR. Eleven (5.0%) variant strains (A(-)B(-)) were detected among the isolates studied and 10 strains (4.5%) had the binary toxin genes (cdtA and cdtB).
引用
收藏
页码:159 / 162
页数:4
相关论文
共 25 条
  • [1] Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile responsible for a nosocomial outbreak of Clostridium difficile-associated diarrhea
    Alfa, MJ
    Kabani, A
    Lyerly, D
    Moncrief, S
    Neville, LM
    Al-Barrak, A
    Harding, GKH
    Dyck, B
    Olekson, K
    Embil, JM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (07) : 2706 - 2714
  • [2] ANTIBIOTIC-ASSOCIATED DIARRHEA
    BARTLETT, JG
    [J]. CLINICAL INFECTIOUS DISEASES, 1992, 15 (04) : 573 - 581
  • [3] BARTLETT JG, 1994, CLIN INFECT DIS S4, V18, P265
  • [4] Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile
    Bidet, P
    Lalande, V
    Salauze, B
    Burghoffer, B
    Avesani, V
    Delmée, M
    Rossier, A
    Barbut, F
    Petit, JC
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (07) : 2484 - 2487
  • [5] LABORATORY DIAGNOSIS OF CLOSTRIDIUM-DIFFICILE-ASSOCIATED DIARRHEA
    BOWMAN, RA
    RILEY, TV
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1988, 7 (04) : 476 - 484
  • [6] SEROGROUP-F STRAINS OF CLOSTRIDIUM-DIFFICILE PRODUCE TOXIN-B BUT NOT TOXIN-A
    DEPITRE, C
    DELMEE, M
    AVESANI, V
    LHARIDON, R
    ROELS, A
    POPOFF, M
    CORTHIER, G
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 1993, 38 (06) : 434 - 441
  • [7] Frequency of binary toxin genes among Clostridium difcile strains that do not produce large clostridial toxins
    Geric, B
    Johnson, S
    Gerding, DN
    Grabnar, M
    Rupnik, M
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (11) : 5227 - 5232
  • [8] Prevalence and characterization of a binary toxin (actin-specific ADP-ribosyltransferase) from Clostridium difficile
    Gonçalves, C
    Decré, D
    Barbut, F
    Burghoffer, B
    Petit, JC
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) : 1933 - 1939
  • [9] Characterization of the enzymatic component of the ADP-ribosyltransferase toxin CDTa from Clostridium difficile
    Gülke, I
    Pfeifer, G
    Liese, J
    Fritz, M
    Hofmann, F
    Aktories, K
    Barth, H
    [J]. INFECTION AND IMMUNITY, 2001, 69 (10) : 6004 - 6011
  • [10] EPIDEMIOLOGY OF COMMUNITY-ACQUIRED CLOSTRIDIUM-DIFFICILE-ASSOCIATED DIARRHEA
    HIRSCHHORN, LR
    TRNKA, Y
    ONDERDONK, A
    LEE, MLT
    PLATT, R
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (01) : 127 - 133