Intercalation interactions between dsDNA and acridine studied by single molecule force spectroscopy

被引：40

作者：

Liu, Chuanjun

Jiang, Zhenhua

Zhang, Yiheng

Wang, Zhiqiang

Zhang, Xi ^{[1
]}

Feng, Fude

Wang, Shu

机构：

[1] Tsinghua Univ, Dept Chem, Key Lab Optoelect & Mol Engn, Beijing 100084, Peoples R China

[2] Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing 100080, Peoples R China

来源：

LANGMUIR | 2007年 / 23卷 / 18期

关键词：

D O I：

10.1021/la7013804

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this letter, we report on the direct measurement of the intercalation interactions between acridine and double-stranded DNA (dsDNA) using single molecule force spectroscopy. The interaction between acridine and dsDNA is broken by force of 36 pN at a loading rate of 5.0 nN/s. The most probable rupture force between acridine and dsDNA is dependent on the loading rate, indicating that the binding of acridine and dsDNA is a dynamic process. The combination of SMFS experimental data with the theoretical model clearly suggests the presence of two energy barriers along with an unbinding trajectory of acridine-dsDNA.

引用

页码：9140 / 9142

页数：3

共 32 条

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