Aggregation of amphiphilic pullulan derivatives evidenced by online flow field flow fractionation/multi-angle laser light scattering

被引：43

作者：

Duval, C ^{[1
]}

Le Cerf, D ^{[1
]}

Picton, L ^{[1
]}

Muller, G ^{[1
]}

机构：

[1] Univ Rouen, CNRS, UMR 6522, PBM, F-76821 Mt St Aignan, France

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 753卷 / 01期

关键词：

derivatization; LC; pullulan;

D O I：

10.1016/S0378-4347(00)00517-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Size-exclusion chromatography (SEC) is a useful steric separation technique for the analysis of water-soluble polysaccharides in aqueous solution. However, in the case of amphiphilic derivatives, the usefulness is limited because of interactions between hydrophobic segments and the stationary phase. Alkyl-bearing pullulans differing from the extent and the length of alkyl groups were characterized using flow-field flow fractionation with on-line coupling multi-angle laser light scattering (F-4/MALLS). Comparison of SEC and F-4 is presented and the interest of F-4 in the field of amphiphilic derivatives is demonstrated. (C) 2001 Elsevier Science BN. All rights reserved.

引用

页码：115 / 122

页数：8

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