Analysis of toxigenic Corynebacterium ulcerans strains revealing potential for false-negative real-time PCR results

被引：21

作者：

Cassiday, Pamela K. ^{[1
]}

Pawloski, Lucia C. ^{[1
]}

Tiwari, Tejpratap ^{[2
]}

Sanden, Gary N. ^{[1
]}

Wilkins, Patricia P. ^{[1
]}

机构：

[1] Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Atlanta, GA 30333 USA

[2] Ctr Dis Control & Prevent, Natl Immunizat Program, Atlanta, GA 30333 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 01期

关键词：

D O I：

10.1128/JCM.01251-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Diphtheria surveillance depends on the rapid and reliable recognition of the toxin gene in Corynebacterium diphtheriae. Real-time PCR is a rapid tool to confirm the presence of the diphtheria toxin gene (tox) in an isolate or specimen. We report that some toxigenic Corynebacterium ulcerans strains show atypical results in a real-time PCR for tox.

引用

页码：331 / 333

页数：3

共 21 条

[1]

*CDCP, 1997, MMWR-MORBID MORTAL W, V46, P330

[2] Characterization of toxigenic Corynebacterium ulcerans strains isolated from humans and domestic cats in the United Kingdom [J].

De Zoysa, A ;

Hawkey, PM ;

Engler, KJ ;

George, R ;

Mann, G ;

Reilly, W ;

Taylor, D ;

Efstratiou, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (09) :4377-4381

[3]

Efstratiou A, 1999, Commun Dis Public Health, V2, P250

[4] Comparison of phenotypic and genotypic methods for detection of diphtheria toxin among isolates of pathogenic corynebacteria [J].

Efstratiou, A ;

Engler, KH ;

Dawes, CS ;

Sesardic, D .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (11) :3173-3177

[5] A modified Elek test for detection of toxigenic corynebacteria in the diagnostic laboratory [J].

Engler, KH ;

Glushkevich, T ;

Mazurova, IK ;

George, RC ;

Efstratiou, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (02) :495-498

[6] Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and b. mallei [J].

Gee, JE ;

Sacchi, CT ;

Glass, MB ;

De, BK ;

Weyant, RS ;

Levett, PN ;

Whitney, AM ;

Hoffmaster, AR ;

Popovic, T .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (10) :4647-4654

[7] NUCLEOTIDE-SEQUENCE OF THE STRUCTURAL GENE FOR DIPHTHERIA-TOXIN CARRIED BY CORYNEBACTERIOPHAGE-BETA [J].

GREENFIELD, L ;

BJORN, MJ ;

HORN, G ;

FONG, D ;

BUCK, GA ;

COLLIER, RJ ;

KAPLAN, DA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (22) :6853-6857

[8]

Hatanaka A, 2003, EMERG INFECT DIS, V9, P752

[9] Laryngopharyngitis by Corynebacterium ulcerans [J].

Kaufmann, D ;

Ott, P ;

Zbinden, R .

INFECTION, 2002, 30 (03) :168-+

[10] Corynebacterium ulcerans in an immunocompromised patient with diphtheria and her dog [J].

Lartigue, MF ;

Monnet, X ;

Le Flèche, A ;

Grimont, PAD ;

Benet, JJ ;

Durrbach, A ;

Fabre, M ;

Nordmann, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (02) :999-1001

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