Pectinesterase and pectin complexes inhibit ion exchange membrane separation

被引:9
作者
Chen, RW [1 ]
Sims, KA [1 ]
Wicker, L [1 ]
机构
[1] Univ Georgia, Dept Food Sci & Technol, Food Proc Res & Dev Lab, Athens, GA 30602 USA
关键词
marsh grapefruit; cloud stability; clarification; pectin; pectinesterase;
D O I
10.1021/jf9707039
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Ion exchange membrane disks (FMC Co., Pine Brook, NJ) equilibrated at pH 5.0, 6.0, 7.2, and 8.0 were tested for separation of pectinesterase (PE) extracted from Marsh grapefruit (MGF) pulp. Anionic exchange disks (QUAT, DEAE, and PEI) bound less PE and protein than cationic exchange disks (SP and CM). Less than 20% of PE was bound to SP membrane at pH 6.0, 7.2, and 8.0. Law PE binding ability in the ion-exchange system may be attributed to the presence of PE-pectin complexes, which act as a competitor with the inorganic ions of the membranes. Water washing the pulp prior to PE extraction lowered the pectin content by 86%, but did not substantially improve PE binding to SP membrane.
引用
收藏
页码:1777 / 1782
页数:6
相关论文
共 36 条
[1]  
[Anonymous], [No title captured]
[2]  
Axelos M.A.V., 1991, The Chemistry and Technology of Pectin, P109, DOI [DOI 10.1016/B978-0-08-092644-5.50011-X, 10.1016/B978-0-08092644-5.50011-X, DOI 10.1016/B978-0-08092644-5.50011-X]
[3]   NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].
BLUMENKR.N ;
ASBOEHAN.G .
ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :484-489
[4]  
BRADDOCK RJ, 1989, ACS SYM SER, V405, P293
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   REGULATION OF PLANT CELL-WALL PECTIN METHYL ESTERASE BY POLYAMINES - INTERACTIONS WITH THE EFFECTS OF METAL-IONS [J].
CHARNAY, D ;
NARI, J ;
NOAT, G .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 205 (02) :711-714
[7]  
DRIOLI E, 1981, ACS SYM SER, V154, P17
[8]   HEAT INACTIVATION TEMPERATURE-TIME RELATIONSHIPS FOR PECTINESTERASE INACTIVATION IN CITRUS JUICES [J].
EAGERMAN, BA ;
ROUSE, AH .
JOURNAL OF FOOD SCIENCE, 1976, 41 (06) :1396-1397
[9]   Pectinesterases from mature, unripe peach fruit bind strongly to pectic polysaccharides [J].
Glover, H ;
Brady, CJ .
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1995, 22 (06) :977-985
[10]  
GOLDBERG B, 1989, AM BIOTECHNOL LAB, V7, P27