Further investigation of the epitope recognized by the new monoclonal antibody 2C9

被引：2

作者：

Kato, K

Yoshikawa, K ^{[1
]}

Taki, T

Shitara, K

Nakamura, K

Hirota, M

Hanai, N

Nakamura, K

Kokubo, H

Mitsui, K

Yamada, Y

Honda, N

Ueda, R

Saga, S

Fukatsu, H

机构：

[1] Aichi Med Univ, Sch Med, Dept Pathol, Aichi 4801195, Japan

[2] Aichi Med Univ, Sch Med, Dept Urol, Aichi 4801195, Japan

[3] Kyowa Hakko Kogyo Co Ltd, Tokyo Res Labs, Tokyo 194, Japan

[4] Nagoya City Univ, Sch Med, Dept Internal Med 2, Nagoya, Aichi 467, Japan

来源：

INTERNATIONAL JOURNAL OF UROLOGY | 2003年 / 10卷 / 08期

关键词：

2C9; prostate cancer; prostate-specific membrane antigen; monoclonal antibody;

D O I：

10.1046/j.1442-2042.2003.00655.x

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: We established a new monoclonal antibody (2C9) that reacted with prostate tissue. The immunohistochemical reactivity of this antibody is similar to anti-prostate-specific membrane antigen (PSMA). Herein, we report the antigenic determinant of 2C9 antibody. Methods: The reactivity of the antibody was characterized by immunohistochemical staining and the antigen target was characterized by amino acid sequencing after immuno-affinity purification from an LNCaP cell lysate and cloning of a cDNA using a mammalian expression cDNA cloning system. Results: The amino acid and nucleotide sequences for the antigen molecule recognized with 2C9 monoclonal antibody demonstrated identity with PSMA. Conclusion: The target molecule of the 2C9 monoclonal antibody is PSMA, pointing to future diagnostic and therapeutic applications.

引用

页码：439 / 444

页数：6

共 22 条

[1] RADIOIMMUNOSCINTIGRAPHY OF PELVIC LYMPH-NODES WITH (111)INDIUM-LABELED MONOCLONAL-ANTIBODY CYT-356 [J].