Ecdysteroids regulate yolk protein uptake by Drosophila melanogaster oocytes

Juvenile hormones (JHs) are thought to drive the regulation of yolk protein uptake by ovaries in Drosophila melanogaster. However, the level of JH production in a mutant stock (ap(56f)) is depressed yet the flies are normally vitellogenic. The production of ecdysteroids by these ap(56f) ovaries in vitro is elevated above that of wild-type ovaries. The incubation of wild-type ovaries in the presence of 0.1 mM JHB(3) increased ecdysteroid biosynthesis only during the first 18 h following eclosion. Female Drosophila melanogaster undergo a pre-vitellogenic reproductive diapause when exposed to low temperature (11 degrees C) and a short-day photoperiod (L12:D12). The rate of ecdysteroid synthesis by the ovaries, but not JH production, increased within 12 h of a temperature upshift to 25 degrees C from a basal level of 20 +/- 1 pg/10 pair of ovaries/5 h to a sustained level of 150 +/- 20 pg/10 pair/5 h. Vitellogenic oocytes were noted in all females within 12 h of this temperature upshift. Diapause was also terminated by the injection of 1 mu g of 20-hydroxyecdysone into the abdomens of diapausing females as determined by an increase in ovary size, and the appearance of vitellogenic oocytes as compared to controls. These results are consistent with a revised model for the regulation of yolk protein uptake by ovaries in which ecdysteroids, and not JHs, play the prominent role. (C) 1998 Elsevier Science Ltd. All rights reserved.