Molecular cloning and characterization of a novel retinoblastoma-binding protein

被引：62

作者：

Fusco, C

Reymond, A

Zervos, AS

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Cutaneous Biol Res Ctr, Charlestown, MA 02129 USA

[2] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Dept Mol Biol, Charlestown, MA 02129 USA

来源：

GENOMICS | 1998年 / 51卷 / 03期

关键词：

D O I：

10.1006/geno.1998.5368

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe the isolation and characterization of a novel cDNA encoding a polypeptide that interacts in a yeast two-hybrid system as well as in mammalian cells with the retinoblastoma (RB) protein. This new protein, which we call Rim, consists of 897 amino acids, has two leucine zipper motifs, and has a LECEE sequence previously identified as an RE-binding domain. Rim also has an E1A/CtBP-binding motif and four putative nuclear localization signals. Rim mRNA is expressed ubiquitously at low levels in all human adult tissues tested and at much higher levels in several tumor cell lines. The Rim gene (HG;MW-approved symbol RBBP8) is localized on human chromosome 18q11.2. (C) 1998 Academic Press

引用

收藏

页码：351 / 358

页数：8

相关论文

共 48 条

[1] THE RETINOBLASTOMA SUSCEPTIBILITY GENE-PRODUCT REPRESSES TRANSCRIPTION WHEN DIRECTLY BOUND TO THE PROMOTER [J].

ADNANE, J ;

SHAO, ZH ;

ROBBINS, PD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (15) :8837-8843

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[3] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[4] ADENOVIRUS E1A PROTEINS CAN DISSOCIATE HETEROMERIC COMPLEXES INVOLVING THE E2F TRANSCRIPTION FACTOR - A NOVEL MECHANISM FOR E1A TRANSACTIVATION [J].

BAGCHI, S ;

RAYCHAUDHURI, P ;

NEVINS, JR .

CELL, 1990, 62 (04) :659-669

[5] ADENOVIRUS-E1A PREVENTS THE RETINOBLASTOMA GENE-PRODUCT FROM COMPLEXING WITH A CELLULAR TRANSCRIPTION FACTOR [J].

BANDARA, LR ;

LATHANGUE, NB .

NATURE, 1991, 351 (6326) :494-497

[6]

Bao JX, 1996, ONCOGENE, V12, P2171

[7] MAX - A HELIX-LOOP-HELIX ZIPPER PROTEIN THAT FORMS A SEQUENCE-SPECIFIC DNA-BINDING COMPLEX WITH MYC [J].

BLACKWOOD, EM ;

EISENMAN, RN .

SCIENCE, 1991, 251 (4998) :1211-1217

[8] A REGION IN THE C-TERMINUS OF ADENOVIRUS-2/5 E1A PROTEIN IS REQUIRED FOR ASSOCIATION WITH A CELLULAR PHOSPHOPROTEIN AND IMPORTANT FOR THE NEGATIVE MODULATION OF T24-RAS MEDIATED TRANSFORMATION, TUMORIGENESIS AND METASTASIS [J].

BOYD, JM ;

SUBRAMANIAN, T ;

SCHAEPER, U ;

LAREGINA, M ;

BAYLEY, S ;

CHINNADURAI, G .

EMBO JOURNAL, 1993, 12 (02) :469-478

[9] Understanding gene and allele function with two-hybrid methods [J].

Brent, R ;

Finley, RL .

ANNUAL REVIEW OF GENETICS, 1997, 31 :663-704

[10] THE RETINOBLASTOMA PROTEIN IS PHOSPHORYLATED DURING SPECIFIC PHASES OF THE CELL-CYCLE [J].

BUCHKOVICH, K ;

DUFFY, LA ;

HARLOW, E .

CELL, 1989, 58 (06) :1097-1105

← 1 2 3 4 5 →