RNA interference for wheat functional gene analysis

被引：59

作者：

Fu, Daolin

Uauy, Cristobal

Blechl, Ann

Dubcovsky, Jorge

机构：

[1] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

[2] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA

来源：

TRANSGENIC RESEARCH | 2007年 / 16卷 / 06期

基金：

美国农业部;

关键词：

wheat; RNA interference; gene silencing;

D O I：

10.1007/s11248-007-9150-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA interference (RNAi) refers to a common mechanism of RNA-based post-transcriptional gene silencing in eukaryotic cells. In model plant species such as Arabidopsis and rice, RNAi has been routinely used to characterize gene function and to engineer novel phenotypes. In polyploid species, this approach is in its early stages, but has great potential since multiple homoeologous copies can be simultaneously silenced with a single RNAi construct. In this article, we discuss the utilization of RNAi in wheat functional gene analysis and its effect on transcript regulation of homoeologous genes. We also review recent examples of RNAi modification of important agronomic and quality traits in wheat and discuss future directions for this technology.

引用

页码：689 / 701

页数：13

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