Putative markers of infective life stages in Leishmania (Viannia) braziliensis

被引:11
作者
Gamboa, D.
Van Eys, G.
Victoir, K.
Torres, K.
Adaui, V.
Arevalo, J.
Dujardin, J. -C.
机构
[1] Intituut Trop Geneeskunde, Unit Mol Parasitol, B-2000 Antwerp, Belgium
[2] Univ Peruana Cayetano Heredia, Inst Med Trop Alexander Humboldt, Lima 100, Peru
[3] Univ Maastricht, Dept Mol Genet, NL-6200 MD Maastricht, Netherlands
[4] Univ Peruana Cayetano Heredia, Fac Ciencias & Filosofia, Dept Bioquim Biol Mol & Farmacol, Lima 100, Peru
关键词
leishmania (Viannia) braziliensis; differential display; gene expression; DIFFERENTIAL GENE-EXPRESSION; IN-VITRO; PROMASTIGOTES; METACYCLOGENESIS; IDENTIFICATION; DONOVANI; HYBRIDIZATION; AMASTIGOTES; RESISTANCE; VIRULENCE;
D O I
10.1017/S003118200700306X
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Gene expression is known to vary significantly during the Leishmania life-cycle. Its monitoring might allow identification of molecular changes associated with the infective stages (metacyclics and amastigotes) and contribute to the understanding of the complex host-parasite relationships. So far, very few studies have been done on Leishmania (Viannia) braziliensis, one of the most pathogenic species. Such studies require, first of all, reference molecular markers. In the present work, we applied differential display analysis (DD analysis) in order to identify transcripts that might be (i) candidate markers of metacyclics and intracellular amastigotes of L. (V.) braziliensis or (ii) potential controls, i.e. constitutively expressed. In total, 48 DNA fragments gave reliable sequencing data, 29 of them being potential markers of infective stages and 12 potential controls. Eight sequences could be identified with reported genes. Validation of the results of DD analysis was done for 4 genes (2 differentially expressed and 2 controls) by quantitative real-time PCR. The infective insect stage-specific protein (meta 1) was more expressed in metacyclic-enriched preparations. The oligopeptidase b showed a higher expression in amastigotes. Two genes, glucose-6-phosphate dehydrogenase and a serine/threonine protein kinase, were found to be similarly expressed in the different biological samples.
引用
收藏
页码:1689 / 1698
页数:10
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