DNA microarray for genotyping multidrug-resistant Pseudomonas aeruginosa clinical isolates

被引：16

作者：

Weile, Jan ^{[1
]}

Schmid, Rolf D.

Bachmann, Till T.

Susa, Milorad

Knabbe, Cornelius

机构：

[1] Robert Bosch Krankenhaus, Dep Lab Med, D-70376 Stuttgart, Germany

[2] Dr Margaret Fisher Bosch Inst Clin Pharmacol, D-70376 Stuttgart, Germany

[3] Univ Stuttgart, Inst Techn Biochem, D-70569 Stuttgart, Germany

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2007年 / 59卷 / 03期

关键词：

Pseudomonas aeruginosa; DNA microarray; antibiotic resistance; nosocomial infections; multidrug resistance;

D O I：

10.1016/j.diagmicrobio.2007.06.005

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The management of infections with multidrug-resistant Pseudomonas aeruginosa needs fast and reliable methods of antibiotic susceptibility testing for a therapy improvement. For this purpose, we developed a DNA microarray for genotyping antibiotic resistance and a few virulence factors. The array covers mutations in the efflux regulators mexR, nfxB, mexT, gyrase gyrA, and parC, as well as plasmid-encoded vim, imp, oxa, aph, aac, and aad genes, and virulence-associated mucA and exoU, exoT, and exoS genes, respectively. The whole procedure can be performed in less than 5 h and consists of DNA isolation, target gene amplification, fluorescence labeling, fragmentation, and array hybridization. Concerning the genotype-phenotype comparison in the test collection, the coverage of relevant resistance determinants for antibiotics used in a calculated therapy of critical ill patients was 87.8%. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：325 / 338

页数：14

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