Size of supramolecular SNARE complex: Membrane-directed self-assembly

被引:56
作者
Cho, WJ [1 ]
Jeremic, A [1 ]
Jena, BP [1 ]
机构
[1] Wayne State Univ, Sch Med, Dept Physiol, Detroit, MI 48201 USA
关键词
D O I
10.1021/ja052442m
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Full length v-SNARE protein in lipid vesicles when exposed to t-SNARE-reconstituted lipid membrane results in the self-assembly of a t-/v-SNARE complex in a ring pattern, forming pores, and establishing continuity between the opposing bilayers. It is known that smaller vesicles fuse more efficiently than larger ones, and hence the curvature of secretory vesicles may dictate the potency and efficacy of their fusion at the cell plasma membrane. The diameter of t- and v-SNARE vesicles may, therefore, reflect the size of the t-/v-SNARE complex formed. In the present study, this hypothesis was tested, and results from the study demonstrate that the size of the t-/v-SNARE complex is directly proportional to the vesicle diameter (R2 = 0.9725). Copyright © 2005 American Chemical Society.
引用
收藏
页码:10156 / 10157
页数:2
相关论文
共 12 条
  • [1] [Anonymous], 1992, INTERMOLECULAR SURFA
  • [2] SNARES in opposing bilayers interact in a circular array to form conducting pores
    Cho, SJ
    Kelly, M
    Rognlien, KT
    Cho, JA
    Hörber, JKH
    Jena, BP
    [J]. BIOPHYSICAL JOURNAL, 2002, 83 (05) : 2522 - 2527
  • [3] Structure, isolation, composition and reconstitution of the neuronal fusion pore
    Cho, WJ
    Jeremic, A
    Rognlien, KT
    Zhvania, MG
    Lazrishvili, I
    Tamar, B
    Jena, BP
    [J]. CELL BIOLOGY INTERNATIONAL, 2004, 28 (10) : 699 - 708
  • [4] G(i) regulation of secretory vesicle swelling examined by atomic force microscopy
    Jena, BP
    Schneider, SW
    Geibel, JP
    Webster, P
    Oberleithner, H
    Sritharan, KC
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (24) : 13317 - 13322
  • [5] Calcium drives fusion of SNARE-apposed bilayers
    Jeremic, A
    Kelly, M
    Cho, JA
    Cho, SJ
    Horber, JKH
    Jena, BP
    [J]. CELL BIOLOGY INTERNATIONAL, 2004, 28 (01) : 19 - 31
  • [6] Vesicle swelling regulates content expulsion during secretion
    Kelly, ML
    Cho, WJ
    Jeremic, A
    Abu-Hamdah, R
    Jena, BP
    [J]. CELL BIOLOGY INTERNATIONAL, 2004, 28 (10) : 709 - 716
  • [7] SMALL-VOLUME EXTRUSION APPARATUS FOR PREPARATION OF LARGE, UNILAMELLAR VESICLES
    MACDONALD, RC
    MACDONALD, RI
    MENCO, BPM
    TAKESHITA, K
    SUBBARAO, NK
    HU, LR
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1061 (02) : 297 - 303
  • [9] Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis
    Schneider, SW
    Sritharan, KC
    Geibel, JP
    Oberleithner, H
    Jena, BP
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (01) : 316 - 321
  • [10] SNAP RECEPTORS IMPLICATED IN VESICLE TARGETING AND FUSION
    SOLLNER, T
    WHITEHART, SW
    BRUNNER, M
    ERDJUMENTBROMAGE, H
    GEROMANOS, S
    TEMPST, P
    ROTHMAN, JE
    [J]. NATURE, 1993, 362 (6418) : 318 - 324