Two-dimensional chromatography in the analysis of complex glycans from transferrin

被引：15

作者：

Charlwood, J ^{[1
]}

Birrell, H ^{[1
]}

Tolson, D ^{[1
]}

Camilleri, P ^{[1
]}

机构：

[1] SmithKline Beecham Pharmaceut, Harlow CM19 5AD, Essex, England

来源：

ANALYTICAL CHEMISTRY | 1998年 / 70卷 / 13期

关键词：

D O I：

10.1021/ac9801059

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Oligosaccharides released from human transferrin have been derivatized with 2-aminoacridone (2-AMAC) prior to analysis by either reversed- or normal-phase highperformance liquid chromatography. These separation methods are complementary and allow distinction between isomeric mono- and disialylated oligosaccharides, which terminate with Sia alpha 2-6Gal or Sia alpha 2-3Gal at the nonreducing end. Collected fractions of 2-AMAC-derivatized glycans were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, before and after desialylation.

引用

页码：2530 / 2535

页数：6

共 20 条

[1]

Adamowicz M, 1996, EUR J PEDIATR, V155, P347

[2]

Anumula Kalyan Rao, 1993, Journal of Molecular Recognition, V6, P139, DOI 10.1002/jmr.300060306

[3] NONSELECTIVE AND EFFICIENT FLUORESCENT LABELING OF GLYCANS USING 2-AMINO BENZAMIDE AND ANTHRANILIC ACID [J].

BIGGE, JC ;

PATEL, TP ;

BRUCE, JA ;

GOULDING, PN ;

CHARLES, SM ;

PAREKH, RB .

ANALYTICAL BIOCHEMISTRY, 1995, 230 (02) :229-238

[4] HIGH-RESOLUTION AND RAPID ANALYSIS OF BRANCHED OLIGOSACCHARIDES BY CAPILLARY ELECTROPHORESIS [J].

CAMILLERI, P ;

HARLAND, GB ;

OKAFO, G .

ANALYTICAL BIOCHEMISTRY, 1995, 230 (01) :115-122

[5] Defective galactosylation of serum transferrin in galactosemia [J].

Charlwood, J ;

Clayton, P ;

Keir, G ;

Mian, N ;

Winchester, B .

GLYCOBIOLOGY, 1998, 8 (04) :351-357

[6] INCOMPLETELY PROCESSED N-GLYCANS OF SERUM GLYCOPROTEINS IN CONGENITAL DYSERYTHROPOIETIC ANEMIA TYPE-II (HEMPAS) [J].

FUKUDA, MN ;

GAETANI, GF ;

IZZO, P ;

SCARTEZZINI, P ;

DELL, A .

BRITISH JOURNAL OF HAEMATOLOGY, 1992, 82 (04) :745-752

[7] A SENSITIVE AND SELECTIVE METHOD FOR THE ANALYSIS OF COMPLEX-MIXTURES OF SUGARS AND LINEAR OLIGOSACCHARIDES [J].

GREENAWAY, M ;

OKAFO, GN ;

CAMILLERI, P ;

DHANAK, D .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1994, (14) :1691-1692

[8] A rapid high-resolution high-performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles [J].

Guile, GR ;

Rudd, PM ;

Wing, DR ;

Prime, SB ;

Dwek, RA .

ANALYTICAL BIOCHEMISTRY, 1996, 240 (02) :210-226

[9] The identification of abnormal glycoforms of serum transferrin in carbohydrate deficient glycoprotein syndrome type I by capillary zone electrophoresis [J].

Iourin, O ;

Mattu, TS ;

Mian, N ;

Keir, G ;

Winchester, B ;

Dwek, RA ;

Rudd, PM .

GLYCOCONJUGATE JOURNAL, 1996, 13 (06) :1031-1042

[10] CARBOHYDRATE-COMPOSITION OF SERUM TRANSFERRIN ISOFORMS FROM PATIENTS WITH HIGH ALCOHOL-CONSUMPTION [J].

LANDBERG, E ;

PAHLSSON, P ;

LUNDBLAD, A ;

ARNETORP, A ;

JEPPSSON, JO .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 210 (02) :267-274

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