Anti-interleukin 8 antibody abolishes effects of lipoid nephrosis cytokine

被引:23
作者
Garin, EH [1 ]
Laflam, P [1 ]
Chandler, L [1 ]
机构
[1] Univ S Florida, Dept Pediat, Div Nephrol, Tampa, FL 33606 USA
关键词
nephrotic syndrome; anti-interleukin 8 neutralizing antibody; albuminuria; (35)sulfate uptake;
D O I
10.1007/s004670050470
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Supernatant factor from peripheral blood mononuclear cell (PBMC) cultures of idiopathic minimal lesion nephrotic syndrome (IMLNS) patients in relapse induces in vivo albuminuria and increases (35)sulfate uptake by glomerular basement membrane (GBM) in rats. The purpose of this study was to evaluate the effect of anti-interleukin 8 (IL8) neutralizing antibody on the effects induced by the supernatant factor. Supernatant factor col lected from six cultures of PBMC from IMLNS patients in relapse were combined and aliquoted into two samples. Anti-IL8 neutralizing antibody (750 ng) was added to one. Supernatant factor or supernatant factor and anti-IL8 antibody were infused for 5 days into the left renal artery of Wistar rats using an osmotic pump. On the last day of infusion, rats were injected with (35)sulfate (1.0 mCi/200 g) intraperitoneally and killed after 8 h. Glomeruli were isolated and GEM obtained. There was a significant increase in (35)sulfate uptake of the infused kidney (169 +/- 52 cpm/mg dry glomerular weight, mean +/- SEM) compared with the uptake seen in the contralateral kidney (116 +/- 41, P < 0.05) when the supernatant factor was infused alone. No significant differences in (35)sulfate incorporation were seen between infused kidney (173 +/- 5) and contralateral kidney (190 +/- 49) when supernatant factor and anti-IL8 antibody were administered. A significant increase in albuminuria was seen on the last day of infusion (0.43 +/- 0.11 albumin/ creatinine ratio, mean) SEM) compared with the ratio prior to infusion of the supernatant factor alone (0.18 +/- 0.03, P <0.05). No significant differences in urinary albumin/creatinine ratios prior to and on the 5th day of infusion were seen when the supernatant factor was administered with anti-IL8 antibody. Supernatant factor effects were abolished by the addition of anti-IL8 neutralizing antibody, suggesting that the described effects are mediated by IL8.
引用
收藏
页码:381 / 385
页数:5
相关论文
共 17 条
[1]  
CHURG J, 1970, LANCET, V1, P1299
[2]  
FISHER ER, 1962, LAB INVEST, V11, P617
[3]  
FONG JSC, 1968, J LAB CLIN MED, V71, P1034
[4]  
GANG NF, 1972, LAB INVEST, V27, P310
[5]   IL-8 PRODUCTION BY PERIPHERAL-BLOOD MONONUCLEAR-CELLS IN NEPHROTIC PATIENTS [J].
GARIN, EH ;
BLANCHARD, DK ;
MATSUSHIMA, K ;
DJEU, JY .
KIDNEY INTERNATIONAL, 1994, 45 (05) :1311-1317
[6]   EFFECT OF LIPOID NEPHROSIS CYTOKINE ON GLOMERULAR SULFATED COMPOUNDS AND ALBUMINURIA [J].
GARIN, EH .
PEDIATRIC NEPHROLOGY, 1995, 9 (05) :587-593
[7]   Effect of interleukin-8 on glomerular sulfated compounds and albuminuria [J].
Garin, EH ;
West, L ;
Zheng, W .
PEDIATRIC NEPHROLOGY, 1997, 11 (03) :274-279
[8]   EFFECT OF LYMPHOKINE FROM NEPHROTIC PERIPHERAL-BLOOD MONONUCLEAR-CELLS ON CATABOLISM OF RAT GLOMERULAR-BASEMENT-MEMBRANE SULFATED COMPOUNDS [J].
GARIN, EH ;
CORONTZES, N .
NEPHRON, 1992, 62 (04) :416-421
[9]   GLOMERULAR DYSFUNCTION IN NEPHROTIC HUMANS WITH MINIMAL CHANGES OR FOCAL GLOMERULOSCLEROSIS [J].
GUASCH, A ;
HASHIMOTO, H ;
SIBLEY, RK ;
DEEN, WM ;
MYERS, BD .
AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (05) :F728-F737
[10]  
MALLICK NP, 1977, CLIN NEPHROL, V7, P87