Inhibitory effects of apple polyphenol on induced histamine release from RBL-2H3 cells and rat mast cells

被引：144

作者：

Kanda, T

Akiyama, H

Yanagida, A

Tanabe, M

Goda, Y

Toyoda, M

Teshima, R

Saito, Y

机构：

[1] Nikka Whisky Distilling Co Ltd, Inst Prod Res & Dev, Kashiwa, Chiba 2770033, Japan

[2] Natl Inst Hlth Sci, Setagaya Ku, Tokyo 1588501, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 07期

关键词：

apple polyphenol; anti-allergic activity; histamine release; hyaluronidase; calcium influx;

D O I：

10.1271/bbb.62.1284

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The anti-allergic activities of polyphenol fractions extracted from immature fruits of apple (Rosaceae, Malus sp.) were evaluated by in vitro assays. A crude apple polyphenol (CAP) fraction, which had been obtained from the juice of immature apples by reverse-phase column chromatography, was further purified by LH-20 column chromatography to obtain an apple condensed tannin (ACT) fraction consisting of linear oligomeric epicatechins from the dimer to pentadecamer. ACT strongly inhibited the release of histamine from rat basophilic leukemia (RBL-2H3) cells stimulated by the antigen-stimulation and from rat peritoneal mast cells stimulated by compound 48/80. The IC50 values for histamine release were 30 mu g/ml and 25 mu g/ml, respectively. ACT also inhibited hyaluronidase activity and the increase in intracellular free calcium concentration in RBL-2H3 cells stimulated with the antigen. These results suggest that ACT affected early signal transduction including the calcium influx.

引用

页码：1284 / 1289

页数：6

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