Delivery of siRNA and siRNA expression constructs to adult mammals by hydrodynamic intravascular injection

被引：39

作者：

Lewis, DL

Wolff, JA

机构：

来源：

RNA INTERFERENCE | 2005年 / 392卷

关键词：

D O I：

10.1016/S0076-6879(04)92020-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Extensive use of RNA interference in mammals has been hindered by the inability to effectively deliver small interfering RNAs (siRNAs) or DNA-based constructs designed to express siRNAs. In this chapter, we describe the high-pressure or hydrodynamic intravascular injection technique used to deliver these nucleic acids to mice and nonhuman primates. Emphasis is placed on the use of this technique for delivery to the liver.

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页码：336 / +

页数：16

相关论文

共 40 条

[1] Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening [J].

Aza-Blanc, P ;

Cooper, CL ;

Wagner, K ;

Batalov, S ;

Deveraux, QL ;

Cooke, MP .

MOLECULAR CELL, 2003, 12 (03) :627-637

[2] A large-scale RNAi screen in human cells identifies new components of the p53 pathway [J].

Berns, K ;

Hijmans, EM ;

Mullenders, J ;

Brummelkamp, TR ;

Velds, A ;

Heimerikx, M ;

Kerkhoven, RM ;

Madiredjo, M ;

Nijkamp, W ;

Weigelt, B ;

Agami, R ;

Ge, W ;

Cavet, G ;

Linsley, PS ;

Beijersbergen, RL ;

Bernards, R .

NATURE, 2004, 428 (6981) :431-437

[3] Short 5′-phosphorylated double-stranded RNAs induce RNA interference in Drosophila [J].

Boutla, A ;

Delidakis, C ;

Livadaras, I ;

Tsagris, M ;

Tabler, M .

CURRENT BIOLOGY, 2001, 11 (22) :1776-1780

[4] Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-κB [J].

Brummelkamp, TR ;

Nijman, SMB ;

Dirac, AMG ;

Bernards, R .

NATURE, 2003, 424 (6950) :797-801

[5] The efficient expression of intravascularly delivered DNA in rat muscle [J].

Budker, V ;

Zhang, G ;

Danko, I ;

Williams, P ;

Wolff, J .

GENE THERAPY, 1998, 5 (02) :272-276

[6]

Budker V, 1996, GENE THER, V3, P593

[7]

Budker V, 2000, J GENE MED, V2, P76, DOI 10.1002/(SICI)1521-2254(200003/04)2:2<76::AID-JGM97>3.0.CO

[8]

2-4

[9] Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems [J].

Caplen, NJ ;

Parrish, S ;

Imani, F ;

Fire, A ;

Morgan, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (17) :9742-9747

[10] A micrococcal nuclease homologue in RNAi effector complexes [J].

Caudy, AA ;

Ketting, RF ;

Hammond, SM ;

Denli, AM ;

Bathoorn, AMP ;

Tops, BBJ ;

Silva, JM ;

Myers, MM ;

Hannon, GJ ;

Plasterk, RHA .

NATURE, 2003, 425 (6956) :411-414

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