The devBCA gene cluster (dev for development), shown to be essential for envelope formation in heterocysts of Anabaena sp. strain PCC 7120, was identified in the gene bank of a second heterocyst-forming strain, Anabaena variabilis ATCC 29413. Sequence and structural organization of the three genes, encoding subunits of a presumptive ABC transporter, were nearly identical in both strains. Mutants of A. variabilis defective in the devA gene were constructed, As devA mutants of Anabaena 7120, A. variabilis mutants were unable to grow on N-2 as sole nitrogen source due to incomplete differentiation of heterocysts. (C) 1998 Elsevier Science B.V. All rights reserved.