Replication-Competent Influenza A Viruses Expressing Reporter Genes

被引:53
作者
Breen, Michael [1 ]
Nogales, Aitor [1 ]
Baker, Steven F. [1 ]
Martinez-Sobrido, Luis [1 ]
机构
[1] Univ Rochester, Dept Microbiol & Immunol, Sch Med & Dent, 601 Elmwood Ave, Rochester, NY 14642 USA
来源
VIRUSES-BASEL | 2016年 / 8卷 / 07期
关键词
recombinant influenza A virus; plasmid-based reverse genetics; virus rescue approaches; reporter genes; fluorescence; luminescence; replicating-competent reporter-expressing influenza A virus; IN-VIVO; PANDEMIC INFLUENZA; LUCIFERASE REPORTER; PACKAGING SIGNALS; NS1; PROTEIN; SIALIC-ACID; INFECTION; TRANSMISSION; GENERATION; SEGMENT;
D O I
10.3390/v8070179
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Influenza A viruses (IAV) cause annual seasonal human respiratory disease epidemics. In addition, IAV have been implicated in occasional pandemics with inordinate health and economic consequences. Studying IAV, in vitro or in vivo, requires the use of laborious secondary methodologies to identify virus-infected cells. To circumvent this requirement, replication-competent IAV expressing an easily traceable reporter protein can be used. Here we discuss the development and applications of recombinant replication-competent IAV harboring diverse fluorescent or bioluminescent reporter genes in different locations of the viral genome. These viruses have been employed for in vitro and in vivo studies, such as the screening of neutralizing antibodies or antiviral compounds, the identification of host factors involved in viral replication, cell tropism, the development of vaccines, or the assessment of viral infection dynamics. In summary, reporter-expressing, replicating-competent IAV represent a powerful tool for the study of IAV both in vitro and in vivo.
引用
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页码:1 / 28
页数:28
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