Hypercholesterolaemia, signs of islet microangiopathy and altered angiogenesis precede onset of type 2 diabetes in the Goto-Kakizaki (GK) rat

被引:22
作者
Giroix, M-H. [1 ]
Irminger, J-C. [2 ]
Lacraz, G. [1 ]
Noll, C. [3 ]
Calderari, S. [4 ]
Ehses, J. A. [5 ,6 ]
Coulaud, J. [1 ]
Cornut, M. [2 ]
Kassis, N. [7 ]
Schmidlin, F. [8 ]
Paul, J-L. [9 ,10 ]
Kergoat, M. [8 ]
Janel, N. [3 ]
Halban, P. A. [2 ]
Homo-Delarche, F. [1 ]
机构
[1] Univ Paris Diderot, Laboratoire Biol & Pathol Pancreas Endocrine B2PE, Unite Biol Fonct & Adaptat BFA EAC CNRS 4413, F-75205 Paris 13, France
[2] Univ Geneva, Dept Genet Med & Dev, CMU, Geneva, Switzerland
[3] Univ Paris Diderot, Lab Deregulat Genique & Differenciat Tris & Hyper, Unite Biol Fonct & Adaptat BFA, F-75205 Paris 13, France
[4] Ctr Rech Cordeliers, Lab Genet & Genom Fonct Insulinoresistance Syst M, Paris, France
[5] Univ British Columbia, Dept Surg, Fac Med, Vancouver, BC V6T 1W5, Canada
[6] Child & Family Res Inst, Vancouver, BC, Canada
[7] Univ Paris Diderot, Lab HERGE Homeostasie Energet & Regulat Nerveuse, Unite Biol Fonct & Adaptat BFA, F-75205 Paris 13, France
[8] Merck Serono, Chilly Mazarin, France
[9] Hop Europeen Georges Pompidou, AH HP, Biochim Lab, Paris, France
[10] Univ Paris 11, Unites Format & Rech UFR Pharm, Chatenay Malabry, France
关键词
Beta cell mass; Cholesterol; Dyslipidaemia; Fetus; Goto-Kakizaki; Islet microangiopathy; Liver; Programming; Type; 2; diabetes; APOLIPOPROTEIN-A-I; LOW-PROTEIN DIET; LIPID-METABOLISM; GENE-EXPRESSION; PARAOXONASE-1; ACTIVITY; VASCULAR DYSFUNCTION; ENDOTHELIAL-CELLS; OXIDATIVE STRESS; INSULIN ACTION; BETA-CELLS;
D O I
10.1007/s00125-011-2223-4
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The adult non-obese Goto-Kakizaki (GK) rat model of type 2 diabetes, particularly females, carries in addition to hyperglycaemia a genetic predisposition towards dyslipidaemia, including hypercholesterolaemia. As cholesterol-induced atherosclerosis may be programmed in utero, we looked for signs of perinatal lipid alterations and islet microangiopathy. We hypothesise that such alterations contribute towards defective pancreas/islet vascularisation that might, in turn, lead to decreased beta cell mass. Accordingly, we also evaluated islet inflammation and endothelial activation in both prediabetic and diabetic animals. Blood, liver and pancreas were collected from embryonic day (E)21 fetuses, 7-day-old prediabetic neonates and 2.5-month-old diabetic GK rats and Wistar controls for analysis/quantification of: (1) systemic variables, particularly lipids; (2) cholesterol-linked hepatic enzyme mRNA expression and/or activity; (3) pancreas (fetuses) or collagenase-isolated islet (neonates/adults) gene expression using Oligo GEArray microarrays targeted at rat endothelium, cardiovascular disease biomarkers and angiogenesis, and/or RT-PCR; and (4) pancreas endothelial immunochemistry: nestin (fetuses) or von Willebrand factor (neonates). Systemic and hepatic cholesterol anomalies already exist in GK fetuses and neonates. Hyperglycaemic GK fetuses exhibit a similar percentage decrease in total pancreas and islet vascularisation and beta cell mass. Normoglycaemic GK neonates show systemic inflammation, signs of islet pre-microangiopathy, disturbed angiogenesis, collapsed vascularisation and altered pancreas development. Concomitantly, GK neonates exhibit elevated defence mechanisms. These data suggest an autoinflammatory disease, triggered by in utero programming of cholesterol-induced islet microangiopathy interacting with chronic hyperglycaemia in GK rats. During the perinatal period, GK rats show also a marked deficient islet vascularisation in conjunction with decreased beta cell mass.
引用
收藏
页码:2451 / 2462
页数:12
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