Different strategies of differential display: areas of application

被引：58

作者：

Matz, MV ^{[1
]}

Lukyanov, SA ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 24期

关键词：

D O I：

10.1093/nar/26.24.5537

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The main goal of this review is to provide some guidelines for choosing a proper method when searching for differentially expressed transcripts. The choice of an approach should depend upon particular features of the experiment and project requirements. We outline some general considerations to help decide between differential display-related techniques and subtractive hybridization. While discussing advantages and problems of differential display, special attention is given to several recently developed methods based on alternative principles of fingerprint production, which provide a possibility for complete systematic investigation of RNA samples.

引用

页码：5537 / 5543

页数：7

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