Profiling of human serum glycans associated with liver cancer and cirrhosis by IMS-MS

被引：126

作者：

Isailovic, D. ^{[1
]}

Kurulugama, R. T. ^{[1
]}

Plasencia, M. D. ^{[1
]}

Stokes, S. T. ^{[1
]}

Kyselova, Z. ^{[1
]}

Goldman, R. ^{[2
]}

Mechref, Y. ^{[1
]}

Novotny, M. V. ^{[1
]}

Clemmer, D. E. ^{[1
]}

机构：

[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

[2] Georgetown Univ, Med Ctr, Dept Oncol, Washington, DC 20057 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2008年 / 7卷 / 03期

关键词：

ion mobility; electrospray ionization; glycans; cancer; conformers; isomers; principal component analysis;

D O I：

10.1021/pr700702r

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aberrant glycosylation of human glycoproteins is related to various physiological states, including the onset of diseases such as cancer. Consequently, the search for glycans that could be markers of diseases or targets of therapeutic drugs has been intensive. Here, we describe a high-throughput ion mobility spectrometry/mass spectrometry analysis of Winked glycans from human serum. Distributions of glycans are assigned according to their m/z values, while ion mobility distributions provide information about glycan conformational and isomeric composition. Statistical analysis of data from 22 apparently healthy control patients and 39 individuals with known diseases (20 with cirrhosis of the liver and 19 with liver cancer) shows that ion mobility distributions for individual m/z ions appear to be sufficient to distinguish patients with liver cancer or cirrhosis. Measurements of glycan conformational and isomeric distributions by IMS-MS may provide insight that is valuable for detecting and characterizing disease states.

引用

页码：1109 / 1117

页数：9

共 41 条

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