The use of real-time PCR to identify Entamoeba histolytica and E-dispar infections in prisoners and primary-school children in Ethiopia

被引:40
作者
Kebede, A
Verweij, JJ
Endeshaw, T
Messele, T
Tasew, G
Petros, B
Polderman, AM
机构
[1] Ethiopian Hlth & Nutr Res Inst, ENARP, Addis Ababa, Ethiopia
[2] Leiden Univ, Med Ctr, Dept Parasitol, NL-2300 RC Leiden, Netherlands
[3] Univ Addis Ababa, Dept Biol, Addis Ababa, Ethiopia
来源
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY | 2004年 / 98卷 / 01期
关键词
D O I
10.1179/000349804225003082
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
In Ethiopia, it is generally unknown what proportion of the amoebic infections commonly found, by microscopy, in humans are caused by non-invasive Entamoeba dispar rather than the potentially invasive E. histolytica. Faecal samples were therefore collected from 363 primary-school students and 409 prisoners from various regions of Ethiopia. Each of these samples was checked for Entamoeba infection by the microscopical examination of formol-ether concentrates. DNA was then extracted from the 213 samples (27.6%) found Entamoeba-positive, and run in a real-time PCR with primers, based on the SSU-rRNA gene sequences of E. histolytica and E. dispar, that allow DNA from the two species to be distinguished. Although E. dispar DNA was identified in 195 (91.5%) of the 213 samples checked by PCR, no E. histolytica DNA was detected. This finding is consistent with the conclusion of a previous, smaller investigation: that many amoebic infections in Ethiopia are incorrectly attributed to E. histolytica and then treated, unnecessarily, with amoebicidal drugs.
引用
收藏
页码:43 / 48
页数:6
相关论文
共 17 条
  • [1] [Anonymous], 1997, Wkly Epidemiol Rec, V72, P97
  • [2] Epidemiology of amebiasis in a region of high incidence of amebic liver abscess in central Vietnam
    Blessmann, J
    Van, LP
    Nu, PAT
    Thi, HD
    Muller-Myhsok, B
    Buss, H
    Tannich, E
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2002, 66 (05) : 578 - 583
  • [3] A REDESCRIPTION OF ENTAMOEBA-HISTOLYTICA SCHAUDINN, 1903 (EMENDED WALKER, 1911) SEPARATING IT FROM ENTAMOEBA-DISPAR BRUMPT, 1925
    DIAMOND, LS
    CLARK, CG
    [J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1993, 40 (03) : 340 - 344
  • [4] Gatti S, 1998, ANN TROP MED PARASIT, V92, P173, DOI 10.1080/00034989860012
  • [5] Entamoeba histolytica and Entamoeba dispar infection in children in Bangladesh
    Haque, R
    Faruque, ASG
    Hahn, P
    Lyerly, DM
    Petri, WA
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (03) : 734 - 736
  • [6] Species-specific field testing of Entamoeba spp. in an area of high endemicity
    Heckendorn, F
    N'Goran, EK
    Felger, I
    Vounatsou, P
    Yapi, A
    Oettli, A
    Marti, HP
    Dobler, M
    Traoré, M
    Lohourignon, KL
    Lengeler, C
    [J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2002, 96 (05) : 521 - 528
  • [7] KEBEDE A, 2003, IN PRESS T ROYAL SOC
  • [8] Kloos H, 1993, ECOLOGY HLTH DIS ETH, P223
  • [9] Niesters HGM, 2002, J CLIN VIROL, V25, pS3
  • [10] Palacz M, 1997, ACTA PHYS POL B, V28, P309