Platelet-rich plasma induces increased expression of G1 cell cycle regulators, type I collagen, and matrix metalloproteinase-1 in human skin fibroblasts

被引:91
作者
Cho, Jae-We [1 ]
Kim, Sung-Ae [1 ]
Lee, Kyu-Suk [1 ]
机构
[1] Keimyung Univ, Sch Med, Dept Dermatol, Taegu 700712, South Korea
关键词
platelet-rich plasma; type I collagen; matrix metalloproteinase; fibroblasts; MAMMARY EPITHELIAL-CELLS; VENOUS ULCERS; SENESCENCE; PROGRESSION; GRANULES;
D O I
10.3892/ijmm.2011.803
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Platelet-rich plasma (PRP) is derived from fresh whole blood, which contains a high concentration of platelets. Recently, PRP has been used for skin wound healing and rejuvenation. However, the molecular mechanisms underlying PRP-inducing wound healing processes are still largely unknown. The aim of this study is to evaluate the effect of PRP on the expression of G1 cell cycle regulatory proteins, type I collagen, matrix metalloproteinase-1 (MMP-1), and MMP-2 in human skin fibroblasts (HSF). We performed a cell proliferation and a migration assay, immunoblotting, and a chloramphenicol acetyltransferase (CAT) assay in PRP-treated human skin fibroblasts. PRP treatment induced increased rates of cell proliferation and cell migration. Expression of cyclin A protein was increased by a low concentration (0.5%) of PRP-treated HSF. In addition, expression of Rb, cyclin E, and cycl in-dependent kinase 4 proteins was increased by a high concentration (5%) of PRP-treated HSF. High concentration of PRP induced an up-regulation of type I collagen, MMP-1, and MMP-2 expression in HSF. Taken together, PRP treatment induced an increase in expression of G1 cell cycle regulators, type I collagen and MMP-1, thereby accelerating the wound healing process.
引用
收藏
页码:32 / 36
页数:5
相关论文
共 22 条
[1]   Platelet-Rich Plasma: A Literature Review [J].
Arora, Navneet S. ;
Ramanayake, Thaminda ;
Ren, Yan-Fang ;
Romanos, Georgios E. .
IMPLANT DENTISTRY, 2009, 18 (04) :303-310
[2]   Autologous platelet-rich plasma as an adipocyte in vivo delivery system:: Case report [J].
Azzena, Bruno ;
Mazzoleni, Francesco ;
Abatangelo, Giovanni ;
Zavan, Barbara ;
Vindigni, Vincenzo .
AESTHETIC PLASTIC SURGERY, 2008, 32 (01) :155-158
[3]   Growth factors and cytokines in wound healing [J].
Barrientos, Stephan ;
Stojadinovic, Olivera ;
Golinko, Michael S. ;
Brem, Harold ;
Tomic-Canic, Marjana .
WOUND REPAIR AND REGENERATION, 2008, 16 (05) :585-601
[4]   Angiogenic properties of sustained release platelet-rich plasma: Characterization in-vitro and in the ischemic hind limb of the mouse [J].
Bir, Shyamal Chandra ;
Esaki, Jiro ;
Marui, Akira ;
Yamahara, Kenichi ;
Tsubota, Hideki ;
Ikeda, Tadashi ;
Sakata, Ryuzo .
JOURNAL OF VASCULAR SURGERY, 2009, 50 (04) :870-879
[5]   Platelet-rich plasma - Clinical applications in dentistry [J].
Carlson, NE ;
Roach, RB .
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION, 2002, 133 (10) :1383-1386
[6]   Regulation of the G1 phase of the mammalian cell cycle [J].
Dubravka, D ;
Scott, DW .
CELL RESEARCH, 2000, 10 (01) :1-16
[7]   ACCUMULATION OF INSULIN-LIKE GROWTH-FACTOR BINDING PROTEIN-3 IN CONDITIONED MEDIUM OF HUMAN FIBROBLASTS INCREASES WITH CHRONOLOGICAL AGE OF DONOR AND SENESCENCE IN-VITRO [J].
GOLDSTEIN, S ;
MOERMAN, EJ ;
BAXTER, RC .
JOURNAL OF CELLULAR PHYSIOLOGY, 1993, 156 (02) :294-302
[8]   COMPARTMENTALIZATION OF A HEMATOPOIETIC GROWTH-FACTOR (GM-CSF) BY GLYCOSAMINOGLYCANS IN THE BONE-MARROW MICROENVIRONMENT [J].
GORDON, MY ;
RILEY, GP ;
WATT, SM ;
GREAVES, MF .
NATURE, 1987, 326 (6111) :403-405
[9]  
Harding Keith G, 2005, Int Wound J, V2, P364, DOI 10.1111/j.1742-4801.2005.00149.x
[10]   PLATELET ALPHA-GRANULES [J].
HARRISON, P ;
CRAMER, EM .
BLOOD REVIEWS, 1993, 7 (01) :52-62