Ultrasound-assisted extraction of flaxseed oil using immobilized enzymes

被引:91
作者
Long, Jing-jing
Fu, Yu-jie [1 ]
Zu, Yuan-gang
Li, Ji
Wang, Wei
Gu, Cheng-bo
Luo, Meng
机构
[1] NE Forestry Univ, Key Lab Forest Plant Ecol, Minist Educ, Harbin 150040, Peoples R China
关键词
Flaxseed oil; Aqueous enzymatic process; Ultrasound extraction; Immobilized enzymes; Antioxidant activity; SEED OIL; PROTEIN;
D O I
10.1016/j.biortech.2011.07.104
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
An aqueous enzymatic process assisted by ultrasound extraction (AEP-UE) was applied to the extraction of oil from flaxseed (Linum usitatissimum L). The highest oil recovery of 68.1% was obtained when ground flaxseed was incubated with 130 U/g of cellulase, pectinase, and hemicellulase for 12 h, at 45 C and pH 5.0. The IC50 values of oil obtained by AEP-UE and organic solvent extraction (OSE), as measured by DPPH scavenging activity essay, were 2.27 mg/mL and 3.31 mg/mL. The AEP-UE-derived oil had a 1.5% higher content of unsaturated fatty acids than the USE-derived oil. AEP-UE is therefore a promising environmentally friendly method for large-scale preparation of flaxseed oil. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:9991 / 9996
页数:6
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