Evaluation of Rapid Antigen Detection Kits for Diagnosis of Equine Rotavirus Infection

被引：10

作者：

Nemoto, Manabu ^{[1
]}

Hata, Hideaki ^{[2
]}

Higuchi, Tohru ^{[2
]}

Imagawa, Hiroshi ^{[1
]}

Yamanaka, Takashi ^{[1
]}

Niwa, Hidekazu ^{[1
]}

Bannai, Hiroshi ^{[1
]}

Tsujmura, Koji ^{[1
]}

Kondo, Takashi ^{[1
]}

Matsumura, Tomio ^{[1
]}

机构：

[1] Japan Racing Assoc, Epizoot Res Ctr, Equine Res Inst, Shimotsuke, Tochigi 3290412, Japan

[2] Hidaka Agr Mutual Aid Assoc, Mitsuishi Anim Clin Ctr, Shinhidaka, Hokkaido 0593105, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2010年 / 72卷 / 09期

关键词：

diagnosis; equine rotavirus; rapid antigen detection; MOLECULAR CHARACTERIZATION; FOALS; AMPLIFICATION; PREVALENCE; SEROTYPE;

D O I：

10.1292/jvms.10-0064

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We evaluated antigen detection kits for human rotavirus with regard to their usefulness for diagnosing equine rotavirus infection. Limiting dilution analyses showed that of the seven kits investigated the Dipstick 'Eiken' Rota (Dipstick) had the highest sensitivity to two serotypes of equine rotavirus. The Dipstick did not cross-react with several equine intestinal pathogens. An investigation using 249 fecal samples indicated that the sensitivity of the Dipstick was 81.9% and 47.3%, and its specificity was 98.2% and 99.0%, and its concordance rate was 92.8% and 68.3%, compared with values obtained using reverse transcription polymerase chain reaction and reverse transcription loop-mediated isothermal amplification, respectively. Although a negative result does not preclude the possibility of equine rotavirus infection, the Dipstick would be useful as routine test for diagnosing equine rotavirus infection in daily clinical practice because of its ease of handling.

引用

页码：1247 / 1250

页数：4

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