The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa:: Cloning, production, and purification of active enzymes

被引：16

作者：

Azzolina, BA ^{[1
]}

Yuan, XL ^{[1
]}

Anderson, MS ^{[1
]}

El-Sherbeini, M ^{[1
]}

机构：

[1] Merck Res Labs, Dept Biochem, Rahway, NJ 07065 USA

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2001年 / 21卷 / 03期

关键词：

bacterial cell wall; cell division; peptidoglycan; Pseudomonads;

D O I：

10.1006/prep.2001.1390

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned the Pseudomonas aeruginosa cell wall biosynthesis and cell division gene cluster that corresponds to the mra operon in the a-min region of the Escherichia coli chromosome. The organization of the two chromosomal regions in P. aeruginosa and E, coli is remarkably similar with the following gene order: pbp3/pbpB, murE, murF, mraY, murD, ftsW, murG, murC, ddlB, ftsQ ftsA, ftsZ, and envA/lpxC, All of the above P. aeruginosa genes are transcribed from the same strand of DNA with very small, if any, intragenic regions, indicating that these genes may constitute a single operon. All five amino acid ligases, MurC, MurD, MurE, MurF, and DdlB, in addition to MurG and MraY were cloned in expression vectors. The four recombinant P. aeruginosa Mur ligases, MurC, MurD, MurE, and MurF were overproduced in E. coli and purified as active enzymes. (C) 2001 Academic Press.

引用

页码：393 / 400

页数：8

共 16 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] INTRACELLULAR STEPS OF BACTERIAL-CELL WALL PEPTIDOGLYCAN BIOSYNTHESIS - ENZYMOLOGY, ANTIBIOTICS, AND ANTIBIOTIC-RESISTANCE [J].