Identification of a membrane receptor for 1,25-dihydroxyvitamin D3 which mediates rapid activation of protein kinase C

被引:168
作者
Nemere, I
Schwartz, Z
Pedrozo, H
Sylvia, VL
Dean, DD
Boyan, BD
机构
[1] Univ Texas, Hlth Sci Ctr, Dept Orthopaed, San Antonio, TX 78284 USA
[2] Utah State Univ, Logan, UT 84322 USA
[3] Hebrew Univ Jerusalem, Hadassah Fac Dent Med, IL-91905 Jerusalem, Israel
关键词
D O I
10.1359/jbmr.1998.13.9.1353
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
This paper is the first definitive report demonstrating a unique membrane receptor for 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3) which mediates the rapid and nongenomic regulation of protein kinase C (PKC). Previous studies have shown that 1,25(OH)(2)D-3 exerts rapid effects on chondrocyte membranes which are cell maturation-specific, do not require new gene expression, and do not appear to act via the traditional vitamin D receptor. We used antiserum generated to a [H-3]1,25(OH)(2)D-3 binding protein isolated from the basal lateral membrane of chick intestinal epithelium (Ab99) to determine if rat costochondral resting zone (RC) or growth zone (GC) cartilage cells contain a similar protein and if cell maturation-dependent differences exist. Immunohistochemistry demonstrated that both RC and GC cells express the protein, but levels are highest in GC. The binding protein is present in both plasma membranes and matrix vesicles and has a molecular weight of 66,000 Da, The 66 kDa protein in GC matrix vesicles has a K-d of 17.2 fmol/ml and B-max of 124 fmol/mg of protein for [H-3]1,25(OH)(2)D-3. In contrast, the 66 kDa protein in RC matrix vesicles has a K-d of 27.7 fmol/ml and a B-max of 100 fmol/mg of protein. Ab99 blocks the 1,25(OH)(2)D-3-dependent increase in PKC activity in GC chondrocytes, indicating that the 1,25(OH)(2)D-3-binding protein is indeed a receptor, linking ligand recognition to biologic function.
引用
收藏
页码:1353 / 1359
页数:7
相关论文
共 50 条
[1]   BINDING CHARACTERISTICS OF A MEMBRANE-RECEPTOR THAT RECOGNIZES 1ALPHA,25-DIHYDROXYVITAMIN D-3 AND ITS EPIMER, 1-BETA,25-DIHYDROXYVITAMIN D-3 [J].
BARAN, DT ;
RAY, R ;
SORENSEN, AM ;
HONEYMAN, T ;
HOLICK, MF .
JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, 56 (04) :510-517
[2]  
Bell R M, 1986, Methods Enzymol, V124, P353
[3]   THE EFFECTS OF VITAMIN-D METABOLITES ON THE PLASMA AND MATRIX VESICLE MEMBRANES OF GROWTH AND RESTING CARTILAGE CELLS-INVITRO [J].
BOYAN, BD ;
SCHWARTZ, Z ;
CARNES, DL ;
RAMIREZ, V .
ENDOCRINOLOGY, 1988, 122 (06) :2851-2860
[4]   DIFFERENTIAL EXPRESSION OF PHENOTYPE BY RESTING ZONE AND GROWTH REGION COSTOCHONDRAL CHONDROCYTES INVITRO [J].
BOYAN, BD ;
SCHWARTZ, Z ;
SWAIN, LD ;
CARNES, DL ;
ZISLIS, T .
BONE, 1988, 9 (03) :185-194
[5]  
Boyan BD, 1997, J CELL BIOCHEM, V66, P457, DOI 10.1002/(SICI)1097-4644(19970915)66:4<457::AID-JCB5>3.0.CO
[6]  
2-K
[7]   NONGENOMIC REGULATION OF EXTRACELLULAR-MATRIX EVENTS BY VITAMIN-D METABOLITES [J].
BOYAN, BD ;
DEAN, DD ;
SYLVIA, VL ;
SCHWARTZ, Z .
JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, 56 (03) :331-339
[8]   INVITRO STUDIES ON THE REGULATION OF ENDOCHONDRAL OSSIFICATION BY VITAMIN-D [J].
BOYAN, BD ;
SCHWARTZ, Z ;
SWAIN, LD .
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE, 1992, 3 (1-2) :15-30
[9]  
BOYAN BD, 1989, CONNECT TISSUE RES, V22, P3
[10]  
BOYAN BD, 1997, VITAMIN D CHEM BIOL, P353