Detection and phylogenetic analysis of norovirus in Corbicula fluminea in a freshwater river in Japan

被引：11

作者：

Saitoh, Mika

Kimura, Hirokazu

Kozawa, Kunihisa

Nishio, Osamu

Shoji, Akira

机构：

[1] Gunma Univ, Dept Biol Sci, Fac Engn, Gunma 3768515, Japan

[2] Gunma Prefectural Inst Publ Hlth & Environm Sci, Gunma 3710052, Japan

[3] Natl Inst Infect Dis, Tokyo 2080011, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2007年 / 51卷 / 09期

关键词：

phylogenetic analysis; norovirus; Corbicula fluminea; freshwater;

D O I：

10.1111/j.1348-0421.2007.tb03977.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To study the molecular epidemiology of noroviruses (NoVs) in bivalves residing in freshwater rivers, we detected, quantified and phylogenetically analyzed the NoV genome in purified concentrates obtained from the gills and digestive diverticula of Corbicula fluminea in a freshwater river in Gunma Prefecture, Japan. We detected the NoV genome in 35 of the 58 C. fluminea samples. Based on our phylogenetic analysis, the NoV genome detected in the samples was classified into 4 genotypes (GI/1, GI/2, GI/3 and GI/4) in genogroup I and 5 genotypes (GII/3, GII/4, GII/5, GII/8 and GII/12) in genogroup II. The phylogenetic tree showed wide genetic diversity among the genogroups. In addition, more than 10(4) copies of the NoV genome were detected in 2 of 35 samples. These results suggest that the freshwater bivalve C. fluminea is a reservoir for NoVs, similar to seawater bivalves such as oysters.

引用

页码：815 / 822

页数：8

共 30 条

[1] DETECTION OF NORWALK VIRUS AND HEPATITIS-A VIRUS IN SHELLFISH TISSUES WITH THE PCR [J].