Identification of an intragenic integration site for foreign gene expression in recombinant Streptococcus gordonii strains

被引：4

作者：

Bolken, TC

Franke, CA

Zeller, GO

Hruby, DE

机构：

[1] SIGA Res Labs, Corvallis, OR 97333 USA

[2] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2001年 / 55卷 / 02期

关键词：

D O I：

10.1007/s002530000519

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new intragenic chromosomal integration site within the lacG gene of the inc operon has been identified in Streptococcus gordonii for use in the expression of foreign genes. Introduction of a portion of the Streptococcus pyogenes emm6 gene into the lacG locus resulted in the lactose-inducible surface expression of the S. pyogenes M6 protein. This result demonstrates the ability to modulate the in vitro or in vivo expression of a foreign gene in a S. gordonii recombinant using a biosynthetic metabolite.

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页码：192 / 197

页数：6

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