The osteogenetic effect of astragaloside IV with centrifugating pressure on the OCT-1 cells

被引:28
作者
Bian, Qin [1 ,2 ,3 ]
Huang, Jian-hua [3 ]
Liang, Qian-qian [1 ,2 ]
Shu, Bing [1 ,2 ]
Hou, Wei [1 ,2 ]
Xu, Hao [1 ,2 ]
Zhao, Yong-jian [1 ,2 ]
Lu, Sheng [1 ,2 ]
Shi, Qi [1 ,2 ]
Wang, Yong-jun [1 ,2 ]
机构
[1] Shanghai Univ Tradit Chinese Med, Inst Spine, Shanghai 200032, Peoples R China
[2] Shanghai Univ Tradit Chinese Med, Longhua Hosp, Dept Orthopaed & Traumatol, Shanghai 200032, Peoples R China
[3] Fudan Univ, Huashan Hosp, Inst Integrated Tradit Chinese Med & Western Med, Shanghai 200433, Peoples R China
来源
PHARMAZIE | 2011年 / 66卷 / 01期
基金
中国国家自然科学基金;
关键词
OSTEOBLAST DIFFERENTIATION; IN-VITRO; RATS; DYSFUNCTION; CBFA1;
D O I
10.1691/ph.2011.0219
中图分类号
R914 [药物化学];
学科分类号
100705 [微生物与生化药学];
摘要
Astragaloside IV (ASI), a pure compound derived from Radix Astragali, is commonly used in degenerative bone diseases such as osteoporosis. Our previous study identified in vivo the osteogenetic effect of Fu Fang Qi She Pills (FFOSP), a Chinese herbal formula containing Radix Astragali from which ASI was extracted. In this study, we investigated the osteogenetic effects of ASI under the conditions of centrifugating pressure on OCT-1 cells. These preosteoblasts were grown in 3D-culture, and treated with ASI at 50 mu mol/l with centrifugation at 200 rpm, 500 rpm for 3 and 5 days. Morphocytological examination, morphometry of alkaline phosphatases (ALP) staining was performed. Expression of type I collagen (Col I) was detected by immunocytochemistry assays. ALP, Col1a2, Osteocalcin (OC), and runt-related transcription factor-2 (Runx2) mRNA expression were determined via real-time PCR. The results showed ASI plus 500 rpm for 3 days and ASI plus 200 rpm for 5 days significantly induced osteogenesis related protein and gene expression. We concluded that ASI would promote osteogenesis when cells of preosteoblast OCT-1 were subjected to proper centrifugating pressure and a pertinent period of time.
引用
收藏
页码:63 / 68
页数:6
相关论文
共 34 条
[1]
TGF-β-induced repression of CBFA1 by Smad3 decreases cbfa1 and osteocalcin expression and inhibits osteoblast differentiation [J].
Alliston, T ;
Choy, L ;
Ducy, P ;
Karsenty, G ;
Derynck, R .
EMBO JOURNAL, 2001, 20 (09) :2254-2272
[2]
[卞琴 BIAN Qin], 2010, [中国脊柱脊髓杂志, Chinese Journal of Spine and Spinal Cord], V20, P311
[3]
Individual osteoblasts in the developing calvaria express different gene repertoires [J].
Candeliere, GA ;
Liu, F ;
Aubin, JE .
BONE, 2001, 28 (04) :351-361
[4]
Neuroprotective effects of Astragaloside IV in 6-hydroxydopamine-treated primary nigral cell culture [J].
Chan, Wing-Sai ;
Durairajan, Siva Sundara Kumar ;
Lu, Jia-Hong ;
Wang, Yan ;
Xie, Li-Xia ;
Kum, Wan-Fung ;
Koo, Irene ;
Yung, Ken Kin Lam ;
Li, Min .
NEUROCHEMISTRY INTERNATIONAL, 2009, 55 (06) :414-422
[5]
Astragaloside IV improves high glucose-induced podocyte adhesion dysfunction via α3β1 integrin upregulation and integrin-linked kinase inhibition [J].
Chen, Jianguo ;
Gui, Dingkun ;
Chen, Yifang ;
Mou, Lijun ;
Liu, Yi ;
Huang, Jianhua .
BIOCHEMICAL PHARMACOLOGY, 2008, 76 (06) :796-804
[6]
Du Q, 2008, CAN J PHYSIOL PHARM, V86, P449, DOI [10.1139/Y08-053, 10.1139/y08-053]
[7]
Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation [J].
Ducy, P ;
Zhang, R ;
Geoffroy, V ;
Ridall, AL ;
Karsenty, G .
CELL, 1997, 89 (05) :747-754
[8]
ASTRAGALOSIDE IV ATTENUATES HYPOXIA-INDUCED CARDIOMYOCYTE DAMAGE IN RATS BY UPREGULATING SUPEROXIDE DISMUTASE-1 LEVELS [J].
Hu, Jiong-Yu ;
Han, Jian ;
Chu, Zhi-Gang ;
Song, Hua-Pei ;
Zhang, Dong-Xia ;
Zhang, Qiong ;
Huang, Yue-Sheng .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2009, 36 (04) :351-357
[9]
Function of the cytoskeleton in gravisensing during spaceflight [J].
Hughes-Fulford, M .
SPACE LIFE SCIENCES: GRAVITATIONAL BIOLOGY: 2002, 2003, 32 (08) :1585-1593
[10]
Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts [J].
Komori, T ;
Yagi, H ;
Nomura, S ;
Yamaguchi, A ;
Sasaki, K ;
Deguchi, K ;
Shimizu, Y ;
Bronson, RT ;
Gao, YH ;
Inada, M ;
Sato, M ;
Okamoto, R ;
Kitamura, Y ;
Yoshiki, S ;
Kishimoto, T .
CELL, 1997, 89 (05) :755-764