Effect of homologous and heterologous prime-boost on the immune response to recombinant plague antigens

被引:41
作者
Glynn, A [1 ]
Freytag, LC [1 ]
Clements, JD [1 ]
机构
[1] Tulane Univ, Hlth Sci Ctr, Program Mol Pathogenesis & Immun, Dept Microbiol & Immunol, New Orleans, LA 70112 USA
关键词
vaccination; plague; Y pestis F1-V;
D O I
10.1016/j.vaccine.2004.10.025
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Among the pathogens that have been identified as potential agents of biological warfare or bioterrorism, Yersinia pestis is one of the main concerns due to the severity and potential transmissibility of the pneurnonic form of the disease in humans. There are no approved vaccines for protection against pneumonic plague, but a Y pestis-derived fusion protein (F1 -V) has shown great promise as a protective antigen in murine studies. In the current study, we examine different prime-boost regimens, including parenteral, mucosal, and transcutaneous delivery, in order to explore the effect of changing the route of prime and boost on the ability of recombinant F1-V to promote the development of long-lasting, high-titer antibodies. The most significant findings of the study reported here are that (1) intranasal and subcutaneous immunizations are both effective and essentially equivalent for induction of serum and bronchioalveolar anti-F1-V IgGI responses when a single booster dose is administered by the same (homologous) route, (2) heterologous boosting can be as or more effective than homologous boosting for induction of either serum or bronchioalveolar anti-F1-V IgGI responses, and (3) anti-F1 and anti-V total IgG responses were highest in animals primed intranasally and boosted by any route when compared to animals primed transcutaneously or subcutaneously. As with previously published studies, there were still significant levels of circulating anti-F1-V antibodies I year post-primary immunization. These studies provide important insights into the development of new-generation biodefense vaccines. (c) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1957 / 1965
页数:9
相关论文
共 54 条
  • [1] Short- and long-term efficacy of single-dose subunit vaccines against Yersinia pestis in mice
    Anderson, GW
    Heath, DG
    Bolt, CR
    Welkos, SL
    Friedlander, AM
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1998, 58 (06) : 793 - 799
  • [2] Intranasal immunization with liposome-formulated Yersinia pestis vaccine enhances mucosal immune responses
    Baca-Estrada, ME
    Foldvari, M
    Snider, M
    Harding, K
    Kournikakis, B
    Babiuk, LA
    Griebel, P
    [J]. VACCINE, 2000, 18 (21) : 2203 - 2211
  • [3] Interplay of cytokines and adjuvants in the regulation of mucosal and systemic HIV-specific CTL
    Belyakov, IM
    Ahlers, JD
    Clements, JD
    Strober, W
    Berzofsky, JA
    [J]. JOURNAL OF IMMUNOLOGY, 2000, 165 (11) : 6454 - 6462
  • [4] Cárdenas-Freytag L, 1999, INFECT IMMUN, V67, P826
  • [5] Chen SC, 1999, ADV POLYM TECH, V18, P1, DOI 10.1002/(SICI)1098-2329(199921)18:1<1::AID-ADV1>3.0.CO
  • [6] 2-0
  • [7] Antibody-independent protection against rotavirus infection of mice stimulated by intranasal immunization with chimeric VP4 or VP6 protein
    Choi, AHC
    Basu, M
    McNeal, MM
    Clements, JD
    Ward, RL
    [J]. JOURNAL OF VIROLOGY, 1999, 73 (09) : 7574 - 7581
  • [8] Functional mapping of protective domains and epitopes in the rotavirus VP6 protein
    Choi, AHC
    Basu, M
    McNeal, MM
    Flint, J
    VanCott, JL
    Clements, JD
    Ward, RL
    [J]. JOURNAL OF VIROLOGY, 2000, 74 (24) : 11574 - 11580
  • [9] LT(R192G), a non-toxic mutant of the heat-labile enterotoxin of Escherichia coli, elicits enhanced humoral and cellular immune responses associated with protection against lethal oral challenge with Salmonella spp.
    Chong, C
    Friberg, M
    Clements, JD
    [J]. VACCINE, 1998, 16 (07) : 732 - 740
  • [10] The F1 and V subunit vaccine protects against plague in the absence of IL-4 driven immune responses
    Elvin, SJ
    Williamson, ED
    [J]. MICROBIAL PATHOGENESIS, 2000, 29 (04) : 223 - 230