Phenylpropanoids as a protectant of aluminum toxicity in cultured tobacco cells

被引:26
作者
Yamamoto, Y
Hachiya, A
Hamada, H
Matsumoto, H
机构
[1] Okayama Univ, Bioresources Res Inst, Kurashiki, Okayama 710, Japan
[2] Okayama Univ Sci, Dept Appl Sci, Okayama 700005, Japan
关键词
aluminum; lipid peroxidation; Nicotiana tabacum; phenylpropanoid; phenylalanine ammonia lyase; phosphate starvation;
D O I
10.1093/oxfordjournals.pcp.a029459
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Aluminum (Al) enhances ferrous ion [Fe(II)]-mediated peroxidation of lipids, which is lethal to normal tobacco cells, but not to phosphate (P-i)-starved cells (-P cells). We found that tobacco cells accumulated phenylpropanoid compounds including chlorogenic acid (CGA) and caffeic acid (CA) during Pi starvation. The accumulation was inhibited by 2-aminoindan-2-phosphonic acid (AIP), a specific inhibitor of L-phenylalanine ammonia lyase (PAL). CGA, CA and also an extract containing the phenylpropanoid compounds from -P cells protected normal cells (+P cells) efficiently from both lipid peroxidation and the loss of viability caused by the combined application of Al and Fe(II), indicating that the phenylpropanoids acted as antioxidant molecules. -P cells exhibited approximately 25-fold higher specific activity of PAL than +P cells. The content of the phenylpropanoids and the activity of PAL were not affected by the combined treatment with Al and Fe(II) in either +P cells or -P cells. These results suggest that an increase in PAL activity during Pi starvation enhances the accumulation of phenylpropanoids, and that the phenylpropanoids protect tobacco cells from cytotoxic lipid peroxidation caused by the combination of Al and Fe(II).
引用
收藏
页码:950 / 957
页数:8
相关论文
共 26 条
[1]   ANTIOXIDANT POTENTIAL OF INTERMEDIATES IN PHENYLPROPANOID METABOLISM IN HIGHER-PLANTS [J].
CASTELLUCCIO, C ;
PAGANGA, G ;
MELIKIAN, N ;
BOLWELL, GP ;
PRIDHAM, J ;
SAMPSON, J ;
RICEEVANS, C .
FEBS LETTERS, 1995, 368 (01) :188-192
[2]   STRESS-INDUCED PHENYLPROPANOID METABOLISM [J].
DIXON, RA ;
PAIVA, NL .
PLANT CELL, 1995, 7 (07) :1085-1097
[3]  
Edwards R., 1992, MOL PLANT PATHOL PRA, V2, P45
[4]   ALUMINUM SALTS ACCELERATE PEROXIDATION OF MEMBRANE-LIPIDS STIMULATED BY IRON SALTS [J].
GUTTERIDGE, JMC ;
QUINLAN, GJ ;
CLARK, I ;
HALLIWELL, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 835 (03) :441-447
[5]  
Halliwell B., 2015, Free radicals in biology and medicine
[6]   THE ROLE OF THE APOPLAST IN ALUMINUM TOXICITY AND RESISTANCE OF HIGHER-PLANTS - A REVIEW [J].
HORST, WJ .
ZEITSCHRIFT FUR PFLANZENERNAHRUNG UND BODENKUNDE, 1995, 158 (05) :419-428
[7]  
IBRAHIM RK, 1987, CELL CULTURE PHYTOCH, V4, P77
[9]   PHOSPHATE MEDIATED REGULATION OF CINNAMOYL PUTRESCINE BIOSYNTHESIS IN CELL-SUSPENSION CULTURES OF NICOTIANA-TABACUM [J].
KNOBLOCH, KH ;
BERLIN, J .
PLANTA MEDICA, 1981, 42 (02) :167-172
[10]   CELLULAR MECHANISMS OF ALUMINUM TOXICITY AND RESISTANCE IN PLANTS [J].
KOCHIAN, LV .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1995, 46 :237-260