Transcription through a simple DNA repeat blocks replication elongation

被引：76

作者：

Krasilnikova, MM ^{[1
]}

Samadashwily, GM ^{[1
]}

Krasilnikov, AS ^{[1
]}

Mirkin, SM ^{[1
]}

机构：

[1] Univ Illinois, Dept Mol Genet, Chicago, IL 60607 USA

来源：

EMBO JOURNAL | 1998年 / 17卷 / 17期

关键词：

elongation; replication; simple sequence repeats; termination; transcription;

D O I：

10.1093/emboj/17.17.5095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of d(G)(n).d(C)(n) repeats on plasmid replication in Escherichia coli cells was analyzed using electrophoretic analysis of replication intermediates. These repeats impeded the replication fork in a length- and orientation-dependent manner. Unexpectedly, the replication arrest relied primarily on the repeats' transcription. When the d(C)(n) sequence served as the transcriptional template, both transcription and replication were blocked. This was true for transcription driven by either bacterial or phage RNA polymerases. We hypothesize that the replication fork halts after it encounters a stalled ternary complex of the RNA polymerase, the DNA template and the r(G)(n) transcript. This constitutes a novel mechanism for the regulation of replication elongation. The effects of this mechanism on repeat length polymorphism and genome rearrangements are discussed.

引用

页码：5095 / 5102

页数：8

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