Analysis of randomly selected cDNAs reveals the expression of stress- and defence-related genes in the barley mutant albostrians

被引：23

作者：

Hess, WR ^{[1
]}

Golz, R ^{[1
]}

Börner, T ^{[1
]}

机构：

[1] Humboldt Univ, Dept Biol, D-10115 Berlin, Germany

来源：

PLANT SCIENCE | 1998年 / 133卷 / 02期

关键词：

ESTs (corticosteroid-binding protein; lipid transfer proteins; 14-3-3; proteins; heat-shock cognate proteins; thiol proteases); Hordeum vulgare L; chloroplast-to-nucleus signal transduction;

D O I：

10.1016/S0168-9452(98)00035-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Randomly selected cDNAs obtained from white leaf material of the albostrians mutant of Hordeum vulgare were analysed. About one third of the cDNAs identified due to their homology to known plant genes are coding for defence-or stress-related proteins. These include several different lipid transfer proteins, an ascorbate peroxidase, a cytochrome P450, a 14-3-3 protein, a thionin, a phenylalanine ammonia lyase, proteases, as well as protein kinases and phosphatases and a heat shock cognate protein. Northern analysis with selected clones showed that the mRNA accumulation in white albostrians leaves differs fundamentally from that in normal green plants. Consequently, the albostrians material provides a unique source of cDNAs only rarely expressed during normal leaf development, and might be of potential value in the identification of novel pathogenesis-and stress-related proteins. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：191 / 201

页数：11

共 58 条

[1] A GENOMIC CLONE ENCODING A PHOSPHOLIPID TRANSFER PROTEIN FROM BARLEY [J].