In vitro conversion of normal prion protein into pathologic isoforms

被引：6

作者：

Bossers, A ^{[1
]}

Rigter, A ^{[1
]}

de Vries, R ^{[1
]}

Smits, MA ^{[1
]}

机构：

[1] Cent Inst Anim Dis Control, NL-8203 AA Lelystad, Netherlands

来源：

CLINICS IN LABORATORY MEDICINE | 2003年 / 23卷 / 01期

关键词：

D O I：

10.1016/S0272-2712(02)00063-X

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

This article describes the various available in vitro systems used to study transmissible spongiform encephalopathy (TSE) replication and the processes involved in prion protein (PrP) conversion. Advantages and disadvantages of these in vitro systems and the underlying molecular mechanisms of PrP conversion are discussed. Several applications of these systems are outlined more extensively, including their species specificity, polymorphism specificity, strain specificity, and the potential that these systems have in screening and discovering TSE therapeutics.

引用

页码：227 / +

页数：23

共 101 条

[1] Conversion of raft associated prion protein to the protease-resistant state requires insertion of PrP-res (PrPSc) into contiguous membranes
Baron, GS
Wehrly, K
Dorward, DW
Chesebro, B
Caughey, B
[J]. EMBO JOURNAL, 2002, 21 (05) : 1031 - 1040
[2] BELG PBG, 1996, BOVINE SPONGIFORM EN, P294
[3] IDENTIFICATION OF 5 ALLELIC VARIANTS OF THE SHEEP PRP GENE AND THEIR ASSOCIATION WITH NATURAL SCRAPIE
BELT, PBGM
MUILEMAN, IH
SCHREUDER, BEC
BOSDERUIJTER, J
GIELKENS, ALJ
SMITS, MA
[J]. JOURNAL OF GENERAL VIROLOGY, 1995, 76 : 509 - 517
[4] DISTINCT PRP PROPERTIES SUGGEST THE MOLECULAR-BASIS OF STRAIN VARIATION IN TRANSMISSIBLE MINK ENCEPHALOPATHY
BESSEN, RA
MARSH, RF
[J]. JOURNAL OF VIROLOGY, 1994, 68 (12) : 7859 - 7868
[5] BIOCHEMICAL AND PHYSICAL-PROPERTIES OF THE PRION PROTEIN FROM 2 STRAINS OF THE TRANSMISSIBLE MINK ENCEPHALOPATHY AGENT
BESSEN, RA
MARSH, RF
[J]. JOURNAL OF VIROLOGY, 1992, 66 (04) : 2096 - 2101
[6] NONGENETIC PROPAGATION OF STRAIN-SPECIFIC PROPERTIES OF SCRAPIE PRION PROTEIN
BESSEN, RA
KOCISKO, DA
RAYMOND, GJ
NANDAN, S
LANSBURY, PT
CAUGHEY, B
[J]. NATURE, 1995, 375 (6533) : 698 - 700
[7] SCRAPIE AND CELLULAR PRION PROTEINS DIFFER IN THEIR KINETICS OF SYNTHESIS AND TOPOLOGY IN CULTURED-CELLS
BORCHELT, DR
SCOTT, M
TARABOULOS, A
STAHL, N
PRUSINER, SB
[J]. JOURNAL OF CELL BIOLOGY, 1990, 110 (03) : 743 - 752
[8] PrP genotype contributes to determining survival times of sheep with natural scrapie
Bossers, A
Schreuder, BEC
Muileman, IH
Belt, PBGM
Smits, MA
[J]. JOURNAL OF GENERAL VIROLOGY, 1996, 77 : 2669 - 2673
[9] Susceptibility of sheep for scrapie as assessed by in vitro conversion of nine naturally occurring variants of PrP
Bossers, A
de Vries, R
Smits, MA
[J]. JOURNAL OF VIROLOGY, 2000, 74 (03) : 1407 - 1414
[10] Scrapie susceptibility-linked polymorphisms modulate the in vitro conversion of sheep prion protein to protease-resistant forms
Bossers, A
Belt, PBGM
Raymond, GJ
Caughey, B
deVries, R
Smits, MA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (10) : 4931 - 4936

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