Was elusive carnivore a panther? DNA typing of faeces reveals the mystery

被引：21

作者：

Verma, SK ^{[1
]}

Prasad, K ^{[1
]}

Nagesh, N ^{[1
]}

Sultana, M ^{[1
]}

Singh, L ^{[1
]}

机构：

[1] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India

来源：

FORENSIC SCIENCE INTERNATIONAL | 2003年 / 137卷 / 01期

关键词：

faecal material; wildlife identification; universal primers; forensics;

D O I：

10.1016/S0379-0738(03)00277-9

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

In this study, we report the findings of a recent case in which the officials of an Indian zoo claimed that an animal, possibly a carnivore, is periodically visiting the zoo from a nearby vast forest area and causing panic in zoo and nearby villages. They collected some elusive faecal material from the vicinity of an herbivore enclosure. Looking to the pugmarks found in that area and faecal matter ceased, the officials could not decide whether it was a lioness, a tiger or a panther. We resolved this mystery by DNA-based analysis of the faecal material, using our recently developed novel universal primers to amplify and sequence a specific fragment of mitochondrial cytochrome b gene. The findings of the DNA-based analyses were confirmed after few days when the zoo officials trapped the animal of same species as suggested in our report. The potential of our procedure to investigate the cases related to wildlife offence is discussed. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：16 / 20

页数：5

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