Structural analysis and in vivo administration of an anti-idiotypic antibody against mAb 2175

被引：11

作者：

Gach, Johannes S. ^{[1
]}

Quendler, Heribert ^{[2
]}

Strobach, Stefanie ^{[1
]}

Katinger, Hermann ^{[1
,2
]}

Kunert, Renate ^{[1
]}

机构：

[1] Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Inst Appl Microbiol, A-1190 Vienna, Austria

[2] Polymun Sci Immunbiol Forschung GmbH, A-1190 Vienna, Austria

来源：

MOLECULAR IMMUNOLOGY | 2008年 / 45卷 / 04期

基金：

奥地利科学基金会;

关键词：

2F5; HIV-1; anti-idiotypic antibodies; vaccine; ELDKWA;

D O I：

10.1016/j.molimm.2007.07.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Anti-idiotypic antibody (Ab2) 3H6 is directed against the human monoclonal antibody 2175, which is one of a few neutralising antibodies against HIV-1. Since the binding epitope of 2175 is cryptic and no neutralising immune response could be elicited by several potential vaccines comprising this region, Ab2/3H6 represents a potent vaccine candidate for active immunisation. Here we describe the molecular features of Ab2/3H6 after changing the antigen binding specificity by single point mutations in the complementarity-determining region 3 of the Ab2/3H6 heavy chain. The resulting Ab2/3H6 mutants were compared in several experimental settings to the wild type Ab2/3H6 Fab fragment. Moreover, we report about an immunisation study with Ab2/3H6 Fab variants, which elicited a specific 2F5-like humoral immune response in BALB/c mice. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1027 / 1034

页数：8

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