Synthesis of the Rheb and K-Ras4B GTPases

被引：63

作者：

Chen, Yong-Xiang ^{[1
,2
]}

Koch, Sebastian ^{[1
,2
]}

Uhlenbrock, Katharina ^{[3
]}

Weise, Katrin

Das, Debapratim ^{[1
,2
]}

Gremer, Lothar ^{[3
]}

Brunsveld, Luc ^{[4
]}

Wittinghofer, Alfred ^{[3
]}

Winter, Roland

Triola, Gemma ^{[1
,2
]}

Waldmann, Herbert ^{[1
,2
]}

机构：

[1] Max Planck Inst Mol Physiol, Abt Chem Biol, D-44227 Dortmund, Germany

[2] Tech Univ Dortmund, Fak Chem, Fachbereich Biol Chem, D-44227 Dortmund, Germany

[3] Max Planck Inst Mol Physiol, Abt Strukturelle Biol, D-44227 Dortmund, Germany

[4] Eindhoven Univ Technol, Biol Chem Lab, NL-5600 MB Eindhoven, Netherlands

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 35期

关键词：

expressed protein ligation; fluorescence spectroscopy; lipoproteins; scanning probe microscopy; solid-phase synthesis; RAS PROTEINS; PLASMA-MEMBRANE; LIPIDATED RAS; DELTA-SUBUNIT; IN-VITRO; N-RAS; MTOR; LOCALIZATION; ASSOCIATION; SIGNALS;

D O I：

10.1002/anie.201001884

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Now available! Farnesylated and carboxymethylated Rheb (see picture) and K-Ras4B GTPases were synthesized in useful amounts by a combination of expressed protein ligation and solid-phase lipopeptide synthesis. The functionality of the proteins was proven by biochemical, biophysical, and cell-based investigations. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：6090 / 6095

页数：6

共 50 条

[1]

Ahmadian Mohammad Reza, 2002, Methods Mol Biol, V189, P45

[2]

Intein-mediated synthesis of geranylgeranylated Rab7 protein in vitro [J].

Alexandrov, K ;

Heinemann, I ;

Durek, T ;

Sidorovitch, V ;

Goody, RS ;

Waldmann, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (20) :5648-5649

[3]

The Rheb family of GTP-binding proteins [J].

Aspuria, PJ ;

Tamanoi, F .

CELLULAR SIGNALLING, 2004, 16 (10) :1105-1112

[4]

Insulin and amino-acid regulation of mTOR signaling and kinase activity through the Rheb GTPase [J].

Avruch, J. ;

Hara, K. ;

Lin, Y. ;

Liu, M. ;

Long, X. ;

Ortiz-Vega, S. ;

Yonezawa, K. .

ONCOGENE, 2006, 25 (48) :6361-6372

[5]

Bioorganic synthesis of lipid-modified proteins for the study of signal transduction [J].

Bader, B ;

Kuhn, K ;

Owen, DJ ;

Waldmann, H ;

Wittinghofer, A ;

Kuhlmann, J .

NATURE, 2000, 403 (6766) :223-226

[6]

Rheb activates mTOR by antagonizing its endogenous inhibitor, FKBP38 [J].

Bai, Xiaochun ;

Ma, Dongzhu ;

Liu, Anling ;

Shen, Xiaoyun ;

Wang, Qiming J. ;

Liu, Yongjian ;

Jiang, Yu .

SCIENCE, 2007, 318 (5852) :977-980

[7]

RAS GENES [J].

BARBACID, M .

ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :779-827

[8]

BOS JL, 1989, CANCER RES, V49, P4682

[9]

Brunsveld L., 2006, ANGEW CHEM, V118, P6774

[10]

Lipidated Ras and Rab peptides and proteins - Synthesis, structure, and function [J].

Brunsveld, Luc ;

Kuhlmann, Juergen ;

Alexandrov, Kirill ;

Wittinghofer, Alfred ;

Goody, Roger S. ;

Waldmann, Herbert .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (40) :6622-6646

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