Circular genomes related to anelloviruses identified in human and animal samples by using a combined rolling-circle amplification/sequence-independent single primer amplification approach

被引：61

作者：

Biagini, Philippe ^{[1
]}

Uch, Rathviro

Belhouchet, Mourad

Attoui, Houssam

Cantaloube, Jean-Francois

Brisbarre, Nadege

de Micco, Philippe

机构：

[1] Serv Virol Mol, Etablissement Francais Sang Alpes Mediterranee, Unite Virus Emergents EA3292, F-13005 Marseille, France

[2] Fac Med, Unite Virus Emergents EA3292, F-13005 Marseille, France

来源：

JOURNAL OF GENERAL VIROLOGY | 2007年 / 88卷

关键词：

D O I：

10.1099/vir.0.83071-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A combined rolling-circle amplification (RCA) and sequence-independent single primer amplification (SISPA) approach was applied to four samples of human plasma and one sample of saliva from a cat. This approach permitted the characterization of nine anelloviruses. Most of them were identified as highly divergent strains that were classified into species of the genus Anellovirus. The smallest anellovirus described so far in humans was characterized (2PoSMA, 2002 nt; 'small anellovirus' species). Two highly divergent sequences belonging to the species Torque Teno Mini Virus (LIL-y1, 2887 nt; LIL-y2, 2871 nt), which clustered into a new phylogenetic branch, were also identified in human plasma samples. Finally, two genomes that are separated by a genetic divergence of 46% were characterized in the cat's saliva, one of these creating a distinct phylogenetic branch (PRA1, 2019 nt). These results highlight the potential of RCA-SISPA for detecting circular (or circularized) genomes.

引用

页码：2696 / 2701

页数：6

共 32 条

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