Naturally Occurring Human Urinary Peptides for Use in Diagnosis of Chronic Kidney Disease

被引：429

作者：

Good, David M. ^{[2
]}

Zuerbig, Petra ^{[1
,7
]}

Argiles, Angel ^{[4
]}

Bauer, Hartwig W. ^{[5
]}

Behrens, Georg ^{[6
]}

Coon, Joshua J. ^{[2
,3
]}

Dakna, Mohammed ^{[1
]}

Decramer, Stephane ^{[10
,11
]}

Delles, Christian ^{[12
]}

Dominiczak, Anna F. ^{[12
]}

Ehrich, Jochen H. H. ^{[9
]}

Eitner, Frank ^{[13
]}

Fliser, Danilo ^{[14
]}

Frommberger, Moritz ^{[15
]}

Ganser, Arnold

Girolami, Mark A. ^{[16
]}

Golovko, Igor ^{[1
]}

Gwinner, Wilfried ^{[8
]}

Haubitz, Marion ^{[8
]}

Herget-Rosenthal, Stefan ^{[17
]}

Jankowski, Joachim ^{[18
]}

Jahn, Holger ^{[19
]}

Jerums, George ^{[20
]}

Julian, Bruce A. ^{[21
,22
]}

Kellmann, Markus ^{[23
]}

Kliem, Volker ^{[24
]}

Kolch, Walter ^{[25
]}

Krolewski, Andrzej S. ^{[26
,27
]}

Luppi, Mario ^{[28
]}

Massy, Ziad ^{[29
,30
,31
]}

Melter, Michael ^{[32
]}

Neusuess, Christian ^{[33
]}

Novak, Jan ^{[21
,22
]}

Peter, Karlheinz ^{[34
]}

Rossing, Kasper ^{[35
]}

Rupprecht, Harald ^{[36
]}

Schanstra, Joost P. ^{[10
]}

Schiffer, Eric ^{[1
]}

Stolzenburg, Jens-Uwe ^{[37
]}

Tarnow, Lise ^{[35
]}

Theodorescu, Dan ^{[38
]}

Thongboonkerd, Visith ^{[39
,40
]}

Vanholder, Raymond ^{[41
]}

Weissinger, Eva M.

Mischak, Harald ^{[1
,12
]}

Schmitt-Kopplin, Philippe ^{[15
]}

机构：

[1] Mosa Diagnost GmbH, D-30625 Hannover, Germany

[2] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA

[3] Univ Wisconsin, Dept Biomol Chem, Madison, WI 53706 USA

[4] RD Nephrol, F-34090 Montpellier, France

[5] Univ Munich, D-80539 Munich, Germany

[6] Hannover Med Sch, Dept Clin Immunol, D-30625 Hannover, Germany

[7] Hannover Med Sch, Dept Hematol Hemostasis Oncol & Stem Cell Transpl, D-30625 Hannover, Germany

[8] Hannover Med Sch, Dept Nephrol, D-30625 Hannover, Germany

[9] Hannover Med Sch, Childrens Hosp, D-30625 Hannover, Germany

[10] INSERM, U858 I2MR, F-31432 Toulouse, France

[11] CHU Toulouse, Hop Enfants, Pediat Nephrol Unit, F-31059 Toulouse, France

[12] Univ Glasgow, British Heart Fdn Glasgow Cardiovasc Res Ctr, Inst Cardiovasc & Med Sci, Coll Med Vet & Life Sci, Glasgow G12 8TA, Lanark, Scotland

[13] RWTH Univ Hosp, Dept Nephrol & Clin Immunol, D-52074 Aachen, Germany

[14] Saarland Univ Hosp, Dept Internal Med 4, D-66421 Homburg, Germany

[15] German Res Ctr Environm Hlth, Helmholtz Ctr Munich, Inst Ecol Chem, D-85764 Neuherberg, Germany

[16] Univ Glasgow, Dept Comp Sci, Glasgow G12 8QQ, Lanark, Scotland

[17] Red Cross Hosp, Dept Med, D-28199 Bremen, Germany

[18] Charite Hosp, Dept Nephrol, D-12200 Berlin, Germany

[19] Univ Hosp Hamburg Eppendorf, Dept Psychiat, D-20246 Hamburg, Germany

[20] Univ Melbourne, Dept Med Austin Hlth & No Hlth, Heidelberg, Vic 3081, Australia

[21] Univ Alabama Birmingham, Dept Microbiol, Birmingham, AL 35294 USA

[22] Univ Alabama Birmingham, Dept Med, Birmingham, AL 35294 USA

[23] Thermo Fisher Sci, D-28199 Bremen, Germany

[24] Lower Saxony Ctr Nephrol, Dept Nephrol, Transplantat Ctr, D-34346 Munden, Germany

[25] Univ Coll Dublin, Dublin 4, Ireland

[26] Joslin Diabet Ctr, Sect Genet & Epidemiol, Boston, MA 02215 USA

[27] Harvard Univ, Sch Med, Dept Med, Boston, MA 02215 USA

[28] Univ Modena & Reggio Emilia, Dept Oncol Hematol & Resp Dis, I-41100 Modena, Italy

[29] INSERM, Dept Clin Pharmacol, ERI 12, F-80054 Amiens, France

[30] INSERM, Dept Nephrol, ERI 12, F-80054 Amiens, France

[31] Univ Picardie Jules Verne, Amiens Univ Hosp, F-80054 Amiens, France

[32] Univ Regensburg, Childrens Hosp, D-93053 Regensburg, Germany

[33] Univ Aalen, Dept Analyt Chem, D-73430 Aalen, Germany

[34] Baker Heart Res Inst, Dept Atherothrombosis & Vasc, Melbourne, Vic 8008, Australia

[35] Steno Diabet Ctr, DK-2820 Gentofte, Denmark

[36] Clin Ctr Bayreuth, Dept Nephrol, D-95444 Bayreuth, Germany

[37] Univ Leipzig, Dept Urol, D-04103 Leipzig, Germany

[38] Univ Virginia, Dept Urol, Charlottesville, VA 22908 USA

[39] Mahidol Univ, Siriraj Hosp, Fac Med, Bangkok 10700, Thailand

[40] Mahidol Univ, Ctr Res Complex Syst Sci, Bangkok 10700, Thailand

[41] Ghent Univ Hosp, Dept Internal Med, B-9000 Ghent, Belgium

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2010年 / 9卷 / 11期

基金：

英国工程与自然科学研究理事会; 美国国家卫生研究院; 英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

ELECTRON-TRANSFER DISSOCIATION; RESOLUTION PROTEOME/PEPTIDOME ANALYSIS; SUPPORT VECTOR MACHINES; TAMM-HORSFALL PROTEIN; MASS-SPECTROMETRY; CAPILLARY-ELECTROPHORESIS; PROTEOMIC ANALYSIS; 2-DIMENSIONAL ELECTROPHORESIS; BIOMARKER DISCOVERY; SEQUENCE-ANALYSIS;

D O I：

10.1074/mcp.M110.001917

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Because of its availability, ease of collection, and correlation with physiology and pathology, urine is an attractive source for clinical proteomics/peptidomics. However, the lack of comparable data sets from large cohorts has greatly hindered the development of clinical proteomics. Here, we report the establishment of a reproducible, high resolution method for peptidome analysis of naturally occurring human urinary peptides and proteins, ranging from 800 to 17,000 Da, using samples from 3,600 individuals analyzed by capillary electrophoresis coupled to MS. All processed data were deposited in an Structured Query Language (SQL) database. This database currently contains 5,010 relevant unique urinary peptides that serve as a pool of potential classifiers for diagnosis and monitoring of various diseases. As an example, by using this source of information, we were able to define urinary peptide biomarkers for chronic kidney diseases, allowing diagnosis of these diseases with high accuracy. Application of the chronic kidney disease-specific biomarker set to an independent test cohort in the subsequent replication phase resulted in 85.5% sensitivity and 100% specificity. These results indicate the potential usefulness of capillary electrophoresis coupled to MS for clinical applications in the analysis of naturally occurring urinary peptides. Molecular & Cellular Proteomics 9:2424-2437, 2010.

引用

页码：2424 / 2437

页数：14

共 73 条

[1]

The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins [J].