Experimental infection of Mongolian gerbils with wild-type and mutant Helicobacter pylori strains

被引:110
作者
Wirth, HP
Beins, MH
Yang, MQ
Tham, KT
Blaser, MJ
机构
[1] Vanderbilt Univ, Sch Med, Med Ctr N A3310, Dept Med,Div Infect Dis, Nashville, TN 37232 USA
[2] Vanderbilt Univ, Sch Med, Dept Pathol, Nashville, TN 37232 USA
[3] Vet Affairs Med Ctr, Nashville, TN 37212 USA
[4] Univ Zurich Hosp, Div Gastroenterol, CH-8091 Zurich, Switzerland
关键词
D O I
10.1128/IAI.66.10.4856-4866.1998
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Experimental Helicobacter. pylori infection a-as studied in Mongolian gerbils with fresh human isolates that early or do not carry cagA (cag4-positive or cagA-negative, respectively), multiply passaged laboratory strains, wild-type strain G1.1, or isogenic ureA, cagA, or vacA mutants of G1.1. Animals were sacrificed 1 to 32 weeks after challenge, the stomach was removed from each animal for quantitative culture, urease test, and histologic testing, and blood was collected for antibody determinations. No colonization occurred after greater than or equal to 20 in vitro passages of wild-type strain G1.1 or with the ureA mutant of G1.1. In contrast, infection occurred in animals challenged with wild-type G1.1 (99 of 101 animals) or the cagA (25 of 25) or vacA (25 of 29) mutant of G1.1. Infection with G1.1 persisted for at least 8 months. All 15 animals challenged with any of three fresh human cagA-positive isolates became infected, in contrast to only 6 (23%) of 26 animals challenged with one of four fresh human cagA-negative isolates (P < 0.001), Similar to infection in humans, H. pylori colonization of gerbils induced gastric inflammation and a systemic antibody response to H, pylori antigens. These data confirm the utility of gerbils as an animal model of H, pylori infection and indicate the importance of bacterial strain characteristics for successful infection.
引用
收藏
页码:4856 / 4866
页数:11
相关论文
共 60 条
[1]   CELLULAR AND HUMORAL IMMUNE-RESPONSES OF JIRDS RESISTANT TO DIPETALONEMA-VITEAE INFECTION [J].
ABRAHAM, D ;
WEINER, DJ ;
FARRELL, JP .
INFECTION AND IMMUNITY, 1986, 52 (03) :742-747
[2]   DNA DIVERSITY AMONG CLINICAL ISOLATES OF HELICOBACTER-PYLORI DETECTED BY PCR-BASED RAPD FINGERPRINTING [J].
AKOPYANZ, N ;
BUKANOV, NO ;
WESTBLOM, TU ;
KRESOVICH, S ;
BERG, DE .
NUCLEIC ACIDS RESEARCH, 1992, 20 (19) :5137-5142
[3]   INABILITY OF AN ISOGENIC UREASE-NEGATIVE MUTANT STRAIN OF HELICOBACTER-MUSTELAE TO COLONIZE THE FERRET STOMACH [J].
ANDRUTIS, KA ;
FOX, JG ;
SCHAUER, DB ;
MARINI, RP ;
MURPHY, JC ;
YAN, LL ;
SOLNICK, JV .
INFECTION AND IMMUNITY, 1995, 63 (09) :3722-3725
[4]   Infection of the ferret stomach by isogenic flagellar mutant strains of Helicobacter mustelae [J].
Andrutis, KA ;
Fox, JG ;
Schauer, DB ;
Marini, RP ;
Li, XT ;
Yan, LL ;
Josenhans, C ;
Suerbaum, S .
INFECTION AND IMMUNITY, 1997, 65 (05) :1962-1966
[5]   DISTRIBUTION AND EFFECTS OF A DEFINED 6-MEMBER MURINE-DERIVED MICROFLORA IN GNOTOBIOTIC GERBILS [J].
BARTIZAL, KF ;
WOSTMANN, BS ;
WAGNER, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 47 (04) :746-751
[6]  
BINGEL SA, 1995, LAB ANIM SCI, V45, P597
[7]   Role of vacA and the cagA locus of Helicobacter pylori in human disease [J].
Blaser, MJ .
ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 1996, 10 :73-77
[8]   cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors [J].
Censini, S ;
Lange, C ;
Xiang, ZY ;
Crabtree, JE ;
Ghiara, P ;
Borodovsky, M ;
Rappuoli, R ;
Covacci, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14648-14653
[9]   THE GERBIL - A UNIQUE MODEL FOR RESEARCH ON AGING [J].
CHEAL, M .
EXPERIMENTAL AGING RESEARCH, 1986, 12 (01) :3-21
[10]   MOLECULAR CHARACTERIZATION OF THE 128-KDA IMMUNODOMINANT ANTIGEN OF HELICOBACTER-PYLORI-ASSOCIATED WITH CYTOTOXICITY AND DUODENAL-ULCER [J].
COVACCI, A ;
CENSINI, S ;
BUGNOLI, M ;
PETRACCA, R ;
BURRONI, D ;
MACCHIA, G ;
MASSONE, A ;
PAPINI, E ;
XIANG, ZY ;
FIGURA, N ;
RAPPUOLI, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5791-5795