Phosphoinositide 3-kinase is required for human adipocyte differentiation in culture

被引：37

作者：

Aubin, D ^{[1
]}

Gagnon, A ^{[1
]}

Sorisky, A ^{[1
]}

机构：

[1] Univ Ottawa, Ottawa Hosp, Ottawa Hlth Res Inst, Dept Med & Biochem Microbiol & Immunol, Ottawa, ON, Canada

来源：

INTERNATIONAL JOURNAL OF OBESITY | 2005年 / 29卷 / 08期

基金：

加拿大健康研究院;

关键词：

adipogenesis; wortmannin; preadipocyte; human;

D O I：

10.1038/sj.ijo.0802961

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

OBJECTIVE: Phosphoinositide 3-kinase (PI3K) is required for murine adipocyte differentiation. However, a recent report concluded that PI3K was not involved in the differentiation of human preadipocytes into adipocytes. We have re-examined the role of PI3K in human preadipocyte differentiation, enrolling more patients and using more adipogenic indices. METHODS: Human preadipocytes, isolated from nine patients, were induced to differentiate in the presence or absence of 100 nM wortmannin. After 12-15 days, triacylglycerol accumulation and the expression of adipogenic markers ( fatty acid synthase and adiponectin) were measured. RESULTS: A significant inhibition in triacylglycerol accumulation and in the induction of fatty acid synthase protein expression was observed, but there was no effect on adiponectin protein expression. CONCLUSION: Inhibition of PI3K reduces the differentiation of human preadipocytes into adipocytes, suggesting a role for this enzyme in the human adipogenic process.

引用

页码：1006 / 1009

页数：4

共 13 条

[1] ADIPSIN/ACYLATION STIMULATING PROTEIN SYSTEM IN HUMAN ADIPOCYTES - REGULATION OF TRIACYLGLYCEROL SYNTHESIS [J].