New beamline dedicated to solution scattering from biological macromolecules at the ESRF

被引：88

作者：

Pernot, P. ^{[1
]}

Theveneau, P. ^{[1
]}

Giraud, T. ^{[1
]}

Fernandes, R. Nogueira ^{[1
]}

Nurizzo, D. ^{[1
]}

Spruce, D. ^{[1
]}

Surr, J. ^{[1
]}

McSweeney, S. ^{[1
]}

Round, A. ^{[2
]}

Felisaz, F. ^{[2
]}

Foedinger, L. ^{[2
]}

Gobbo, A. ^{[2
]}

Huet, J. ^{[2
]}

Villard, C. ^{[2
]}

Cipriani, F. ^{[2
]}

机构：

[1] ESRF, BP 220, Grenoble, France

[2] EMBL Grenoble, Grenoble, France

来源：

XIV INTERNATIONAL CONFERENCE ON SMALL-ANGLE SCATTERING (SAS09) | 2010年 / 247卷

关键词：

D O I：

10.1088/1742-6596/247/1/012009

中图分类号：

O7 [晶体学];

学科分类号：

070301 [无机化学];

摘要：

The new bio-SAXS beamline (ID14-3 at the ESRF, Grenoble, France) is dedicated exclusively to small-angle scattering experiments of biological macromolecules in solution and has been in user operation since November 2008. Originally a protein crystallography beamline, ID14-3 was refurbished, still as a part of the ESRF Structural Biology group, with the main aim to provide a facility with 'quick and easy' access to satisfy rapidly growing demands from crystallographers, biochemists and structural biologists. The beamline allows manual and automatic sample loading/unloading, data collection, processing (conversion of a 2D image to a normalized 1D X-ray scattering profile) and analysis. The users obtain on-line standard data concerning the size (radius of gyration, maximum dimension and volume) and molecular weight of samples which allow on-the fly ab-inito shape reconstruction in order to provide feedback enabling the data collection strategies to be optimized. Automation of sample loading is incorporated on the beamline using a device constructed in collaboration between the EMBL (Grenoble and Hamburg outstations) and the ESRF. Semi/automated data analysis is implemented following the model of the SAXS facility at X33, EMBL Hamburg. This paper describes the bio-SAXS beamline and set-up characteristics together with the examples of user data obtained.

引用

页数：8

共 3 条

[1]

Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2 [J].