Activation of p38 has opposing effects on the proliferation and migration of endothelial cells

被引:119
作者
McMullen, ME
Bryant, PW
Glembotski, CC
Vincent, PA
Pumiglia, KM
机构
[1] Albany Med Coll, Ctr Cell Biol & Canc Res, Albany, NY 12208 USA
[2] Albany Med Coll, Ctr Cardiovasc Sci, Albany, NY 12208 USA
[3] San Diego State Univ, Inst Heart, San Diego, CA 92182 USA
[4] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA
关键词
D O I
10.1074/jbc.M407060200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Pathological conditions such as hypertension and hyper-glycemia as well as abrasions following balloon angioplasty all lead to endothelial dysfunction that impacts disease morbidity. These conditions are associated with the elaboration of a variety of cytokines and increases in p38 activity in endothelial cells. However, the relationship between enhanced p38 activity and endothelial cell function remains poorly understood. To investigate the effect of enhanced p38 MAPK activity on endothelial cell function, we expressed an activated mutant of MEK6 (MEK6E), an upstream regulator of p38. Expression of MEK6E activated p38 and resulted in phosphorylation of its downstream substrate, heat shock protein 27 (Hsp27). Activation of p38 was not sufficient to induce apoptosis; however, it did induce p38-dependent cell cycle arrest. MEK6E expression was sufficient to inhibit ERK phosphorylation triggered by growth factors and integrin engagement. MAPK phosphatase-1 (MKP-1) expression was increased upon p38 activation, and expression of a "substrate-trapping" MKP-1 was sufficient to restore ERK activity. Activation of p38 was sufficient to induce cell migration, which was accompanied by alterations in actin architecture characterized by enhanced lamellipodia. Co-expression of a mutant form of Hsp27, lacking all three phosphorylation sites, reversed MEK6E-induced cell migration and altered the cytoskeletal changes induced by p38 activation. Collectively, these results suggest that cellular decisions regarding migration and proliferation are influenced by p38 activity and that prolonged activation of p38 may result in an anti-angiogenic phenotype that contributes to endothelial dysfunction.
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页码:20995 / 21003
页数:9
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