Culturing of human peripheral blood cells reveals unsuspected lymphocyte responses relevant to HIV disease

被引：33

作者：

Sahaf, Bita ^{[1
]}

Atkuri, Kondala ^{[1
]}

Heydari, Kartoosh ^{[2
]}

Malipatlolla, Meena ^{[1
]}

Rappaport, Jay ^{[3
]}

Regulier, Emmanuel ^{[3
]}

Herzenberg, Leonard A. ^{[1
]}

Herzenberg, Leonore A. ^{[1
]}

机构：

[1] Stanford Univ, Dept Genet, Sch Med, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Pediat, Sch Med, Div Stem Cell Transplantat, Stanford, CA 94305 USA

[3] Temple Univ, Sch Med, Dept Neurosci, Philadelphia, PA 19122 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2008年 / 105卷 / 13期

关键词：

cell culture; HIV infection; HIV-Tat; PBMC;

D O I：

10.1073/pnas.0712363105

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recombinant HIV-Tat (Tat) induces extensive apoptosis in peripheral blood mononuclear cells (PBMCs) cultured in typical CO2 incubators, which are equilibrated with air (21% O-2). However, as we show here, Tat apoptosis induction fails in PBMCs cultured at physiological oxygen levels (5% O-2)- Under these conditions, Tat induces PBMCs to divide, efficiently primes them for HIV infection, and supports virus production by the infected cells. Furthermore, Tat takes only 2 h to prime PBMCs under these conditions. In contrast, PHA/IL-2, which is widely used to prime cells for HIV infection, takes 2-3 days. These findings strongly recommend culturing primary cells at physiological oxygen levels. In addition, they suggest HIV-Tat as a key regulator of HIV disease progression.

引用

页码：5111 / 5116

页数：6

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