Graves' IgG activate upstream enhancer of the sodium/iodide symporter

被引：4

作者：

Breous, E ^{[1
]}

Wenzel, A ^{[1
]}

Loos, U ^{[1
]}

机构：

[1] Univ Ulm, Innere Med Abt 1, D-89081 Ulm, Germany

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2003年 / 213卷 / 01期

关键词：

Graves' disease; TSH receptor autoantibodies; sodium/iodide symporter; regulatory elements;

D O I：

10.1016/j.mce.2003.10.039

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

Graves' thyroid tissue has been shown to express elevated levels of human sodium/iodide symporter (hNIS) mRNA and protein. In the present work, we demonstrate for the first time that hNIS gene expression in Graves' disease (GD) is up-regulated by Graves' IgG. Here, in transient transfection experiments using FRTL-5 cells, hNIS promoter and enhancer/luciferase construct showed an up to six-fold increase in transcriptional activity after incubation with purified Graves' IgG. Mutation of a CRE site in hNIS enhancer inhibited Graves' IgG response. In addition, mutation of a novel putative regulatory region in hNIS promoter reduced the stimulation three-fold. This discovered putative regulatory sequence might play a role in hNIS up-regulation by Graves' IgG and TSH. The data presented here complement our current knowledge of the pathogenesis of GD and will contribute to a better understanding of mechanisms regulating the thyroid iodide concentrating activity. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：109 / 113

页数：5

共 23 条

[1]

Regulation and tissue distribution of the human sodium iodide symporter gene [J].